苯甲酰化褐藻多糖对PC12细胞存活率的影响

EFFECT OF PHTHALANDIONE FUCOIDAN ON CELL VIABILITY OF PC12 CELLS

目的通过观察苯甲酰化褐藻多糖(PHF)对PC12细胞存活率的影响,探讨PHF对神经细胞的作用。方法采用四甲基偶氮唑盐(MTT)方法,检测不同浓度PHF作用下PC12细胞的存活率。结果低剂量的PHF(0.001、0.010g/L)对PC12细胞的存活率没有影响;较高剂量的PHF(0.100、1.000、10.000g/L)可以显著降低PC12细胞的存活率,10.000g/L PHF处理组细胞的存活率下降最明显。结论低剂量的PHF不影响PC12细胞的存活率,而较高浓度的PHF能显著降低PC12细胞的存活率,产生剂量依赖性的毒性作用。

Objective To investigate the influence of phthalandione fucoidan （PHF） on PC12 cell viability and the action of PHF on the cells. Methods MTT assay was used to detect the effect of different-concentration PHF on survival rate of PC12 cells. Results Lower doses of PHF （0.001 g/L and 0.010 g/L） had no effect on the cell viability. However, higher doses of PHF （0.100,1.000,10.000 g/L） could significantly reduce the cell viability, the most reduction was noted in the group of 10.000 g/ L PHF. Conclusion I.ow doses of PHF do not affect the survival of PCl2 cells, and high doses result in dose-dependent toxic effect.