摘要
目的观察刺参酸性黏多糖(SJAMP)体外诱导人肝癌细胞HepG2凋亡的情况,探讨SJAMP对HepG2细胞线粒体凋亡途径相关膜电位作用机制。方法体外培养人肝癌细胞HepG2,用不同浓度的SJAMP(0.25、1.00、4.00mg/L)对其进行干预,应用流式细胞仪检测细胞线粒体膜电位改变,Fura-2荧光显微镜检测细胞钙离子浓度改变。在相同条件下干预人正常肝细胞(张氏细胞)以观察其对正常细胞的影响。结果随着SJAMP剂量的增加,HepG2细胞内钙离子浓度及线粒体膜电位均降低,各干预组与空白对照组比较、各干预组间比较差异均有显著性(F=183.36、264.24,q=3.26～35.67,P<0.05);而张氏细胞各干预组钙离子浓度及线粒体膜电位(除0.25mg/L SJAMP作用组线粒体膜电位高于其他组外)差异无统计学意义(P>0.05)。结论 SJAMP可能通过降低细胞内钙离子浓度和细胞线粒体膜电位,诱导线粒体通透性的改变,启动细胞凋亡途径。
Objective To study the HepG2 cell apoptosis induced by Stichopus japonicus acidic mucopolysaccharide （SJAMP） in vitro, and explore the mechanism of changes of its correlated mitochondrial membrane potential. Methods HepG2 cells cultured in vitro were exposed to different concentrations of SJAMP （0.25, 1.00, 4.00 mg/L）. Flow cytometry was used to detect the changes of mitochondrial membrane potential, and a fluorescence microscope to measure the calcium ion concentration in the cells. Changes liver cells were interfered under the same conditions to observe their influence on normal cells. Results With the increasing of SJAMP dose, the intracellular calcium ion concentration and membrane potential decreased, a comparison between each intervention group and blank control group, as well as between each intervention group, the differences being significant （F= 183.36,264.24;q=3.26-35.67;P〈0.05）. In Chang＇s liver cells, the differences of calcium ion concentrations and membrane po- tential between each intervention group were not significant-except 0.25 mg/L SJAMP action group （P〉0.05）. Conclusion SJAMP is likely through decreasing intracellular calcium ion concentration and cell mitochondrial membrane potential, inducing changes of permeability of mitochondrial membrane and initiating the path of apoptosis.
出处
《齐鲁医学杂志》
2013年第4期283-285,288,共4页
Medical Journal of Qilu
基金
国家自然基金资助项目(002-002395)
