CTLA-4基因CT60位点基因多态性与小儿急性淋巴细胞白血病相关性

ASSOCIATION OF CYTOTOXIC T LYMPHOCYTE ANTIGEN-4 CT60 GENE POLYMORPHISM WITH ACUTE LYMPHOBLASTIC LEUKEMIA IN CHILDREN

目的探讨CTLA-4基因CT60位点多态性与小儿B系急性淋巴细胞白血病(ALL)的关系。方法选取86例B系ALL病儿和112例正常对照者,分别留取外周血标本,提取基因组DNA,聚合酶链式反应及产物直接测序方法检测CT60位点基因型,比较两组以及病例组不同性别、不同临床危险度病儿基因型频率及等位基因频率的差异。结果病例组CT60位点AA和AG基因型频率显著低于对照组(χ2=6.365,P<0.05),A等位基因频率亦明显低于对照组(χ2=6.761,P<0.05);CT60位点基因型及等位基因频率分布与B系ALL病儿性别及临床危险度无关(P>0.05)。结论 CTLA-4基因CT60位点基因多态性与小儿B系ALL易感性存在相关性。

Objective To investigate the correlation between cytotoxic T lymphocyte antigen 4 （CTLA-4） CT60 gene polymorphism and children with acute lymphoblastie leukemia （ALL）. Methods A total of 86 children with ALL and 112 healthy controls was selected. Their genomic DNA was extracted from peripheral blood samples. The genotype was determined by polyrnerase chain reaction （PCR） and PCR product sequencing. Genotype and alleles frequency were compared between the two groups. Results The frequency of AA and AG genotype was significantly lower in the patients than the controls （Х^2 =6.365, P〈0.05）, and so did A allele gene （Х^2=6.761, P〈 0.05）. This kind of difference was not associated with sex and clinical risk. Conclusion CTLA-4 CT60 gene polymorphism is associated with susceptibility to ALL in children.