塞来昔布对胰腺癌细胞株SW1990增殖及干性标记物CD_(133)表达的影响

THE INFLUENCE OF CELECOXIB ON PROLIFERATION OF PANCREATIC CANCER CELL LINE SW1990 AND EXPRESSION OF STEM CELL MARKER CD_(133)

目的观察环氧合酶-2(COX-2)选择性抑制剂塞来昔布对胰腺癌细胞株SW1990增殖及肿瘤干细胞标记物CD133表达的影响。方法取对数生长期的SW1990细胞,分别加入不同浓度的单药吉西他滨、单药塞来昔布、塞来昔布联合吉西他滨进行培养,观察SW1990细胞增殖抑制情况及CD133mRNA、表面分子CD133表达变化。结果不同浓度塞来昔布与吉西他滨(0.500μmol/L)联合应用,对SW1990细胞的增殖抑制作用更为明显,与0.500μmol/L吉西他滨组比较,差异均有显著性(F=339.28～967.22,q=4.48～36.76,P<0.05),与对应剂量的塞来昔布组比较,差异亦均有显著性(q=16.24～66.23,P<0.05)。不同浓度塞来昔布组胰腺癌细胞株SW1990CD133mRNA的表达较对照组明显下调(F=51.84～625.80,q=14.39～45.03,P<0.05),联合应用塞来昔布与吉西他滨(0.500μmol/L)时,其下调作用较各浓度单药塞来昔布减弱,差异有显著性(q=2.85～7.75,P<0.05)。结论塞来昔布对胰腺癌细胞株SW1990的增殖有抑制作用,且与吉西他滨有协同作用;塞来昔布可显著下调SW1990细胞株干细胞表面标记物CD133mRNA的表达。

Objective To observe the effect of eyclooxygenase-2 （COX-2） selective inhibitor celecoxib on pancreatic cancer cell line SW1990 proliferation and stem cell marker CD133 expression. Methods The SW1990 cells in logarithmic growth phase were cultured with different concentrations of gemcitabine, celecoxib and celeqoxib combined with gemcitabine, respectively. The suppression of SW1990 proliferation, and mRNA of CD133 were observed. Results In combined celecoxib with gemcitabine group, the proliferation of SW1990 cells was more obvious as compared with gemcitabine group （0.500μmol/L）, the difference between the two groups were significant （F=339.28-967.22;q=4.48 36.76;P^0.05）, the difference was also significant as compared with celecoxib group of corresponding dose （q=16.24-66.23,P〈0.05）. The expression of SW1990 CD133 mRNA in cele coxib group was lower than that in the control （F=51.84-625.80 ;q=14.39-45.03 ;P〈0.05）, in combined group, its down-re gulated action was weaker than that in different-dose celecoxib groups, the differences being statistically significant （q = 2.85- 7.75,P〈0.05）. Conclusion Celecoxib inhibits the proliferation of SW1990, and has a joint action with gemcitabine. Celecoxib can dramatically down regulate the expression of stem cell surface marker CD133mRNA.