糖尿病大鼠心肌组织内脏脂肪素的表达及二甲双胍干预作用的观察

The expression of visfatin in myocardium of diabetic rats and the effect of metformin on the expression

目的探讨糖尿病大鼠心肌组织内脏脂肪素(Visfatin)、磷脂酰肌醇酶-3激酶(PI3K)表达及二甲双胍对其干预的作用。方法 60只SD大鼠随机分为正常对照(NC)组、糖尿病(DM)组、二甲双胍治疗(M+DM)组,成模后测定FBG、FIns、FFA、TG、TC、LDL-C,计算胰岛素抵抗指数(HOMA-IR)、ISI;测定心肌Visfatin、PI3K mRNA表达及Visfatin蛋白表达;观察心肌病理变化。结果 DM组FBG、FFA、TG、TC、FIns、HOMA-IR较NC组增高(P<0.05),M+DM组较DM组降低(P<0.05)。DM组心肌组织Visfatin、PI3K mRNA表达较NC组降低(P<0.05),M+DM组心肌组织Visfatin、PI3KmRNA表达均较DM组增高(P<0.05);M+DM组心肌组织Visfatin蛋白表达较DM组增高(P<0.05)。结论二甲双胍可提高糖尿病大鼠心肌组织Visfatin、PI3K表达,对心肌组织保护的作用机制可能是通过调节心肌Visfatin表达水平进而通过PI3K途径完成。

Objective To observe the expressions of visfatin and phosphoinositide 3-kinase （PI3K） in the myocardium of diabetic rats and the their changes of under metformin intervention. Methods Sixty male Sprague-Dawley rats were randomized into the normal control （NC）group, diabetes （DM） group and metformin treatment （M＋DM） group. The serum levels of FBG, FFA, TG, TC, LDL-C, and Fins of diabetic models were measured to calculate HOMA-IR and ISI, the expressions of visfatin and PI3K mRNA in the myocardium were determined, the protein expression of visfatin in the myocardium was detected by immunhistochemical stain, and the pathological changes in the myocardium of rats were observed. Results The levels of FBG, FFA, TG, TC, Fins, and HOMA-IR were significantly higher in DM group than in NC group （P〈0. 05）, as compared with the DM group, those levels in the M＋DM group were decreased （P〈0. 05）. The expressions of visfatin and PI3K mRNA in the myocardium was lower in the DM group than in NC group （P〈0. 05） ,and were higher in M＋DM than in DM group （P〈 0.05）. The expression of visfatin was higher in M＋DM group than in DM group （P〈0.05）. Conclusion Metformin functions to increase the expressions of visfatin and PI3K in the myocardium of DM rats. Its protective functions for the myocardium are probably realized through regulating the expression of visfatin in the myocardium via the PI3K pathway.