枸杞多糖对2型糖尿病大鼠周围神经病变的保护作用

Protective effect of lycium barbarum polysaccharides on peripheral neuropathy in type 2 diabetic rats

目的探讨枸杞多糖对T2DM大鼠周围神经病变的保护作用及其机制。方法 SD大鼠制备成T2DM大鼠模型后,随机分为模型组、枸杞多糖小剂量组、大剂量组及α-硫辛酸对照组。治疗10周后检测各组坐骨神经的传导速度,并测该组织中丙二醛(MDA)及谷胱甘肽过氧化酶物(GSH-Px)含量,观察其超微结构。结果与模型组相比,枸杞多糖大剂量组能改善神经传导速度[(32.31±3.54)vs(27.11±5.99)m/s,P<0.05],降低坐骨神经组织中MDA水平[(1.80±1.03)vs(2.32±1.02)nmol/ml,P<0.05],提高GSH-Px水平[(107.44±16.03)vs(67.83±28.91)U/gprot,P<0.05]。模型组坐骨神经超微结构有明显的损伤反应,经枸杞多糖干预后病变程度减轻。结论枸杞多糖通过抗氧化应激,可以提高神经传导速度,并同时改善周围神经的结构和功能,具有保护周围神经病变的作用。

Objective To explore the protective effect of lycium barbarum polysaccharides （LBP） on peripheral neuropathy in type 2 diabetic rats and its mechanism. Methods SD rats with STZ-induced T2DM were randomly divided into the model group, LBP low- and high-dose groups, and α-lipoic acid group. The course of treatment lasted for 10 weeks. The conduction velocity of sciatic nerve of all groups was detected and the contents of MDA and GSH-Px in sciatic nerve were determined, and the changes in its ultrastructure were observed. Results Compared with the model group, the LBP high dose group accelerated the nerve conduction velocity [（32. 31±3.54） vs （27. 11±5. 99） m/s,P〈0. 05], attenuated the content of MDA in sciatic nerve [（1. 80＋1.03） vs （2. 324-1.02） nmol/ml, P〈0. 05], and elevated the content of GSH-Px I-（107.44±16.03） vs （67. 83±28. 91） U/gprot, P〈0. 05]. There were obvious damage reactions in the ultrastructure of sciatic nerve in the model group. However, the pathological condition in sciatic nerve was alleviated after the intervention with LBP. Conclusion LBP accelerates damages of the nerve conduction velocity through anti-oxidative stress, at the same time, it ameliorates damages of the structure and function of peripheral nerves, thus protecting the type 2 diabetic rats with peripheral neuropathy.