增强UV-B辐射对小麦TaRAN1蛋白分布与定位的影响

Enhanced UV-B Radiation on Wheat TaRAN1 Protein Distribution and the Influence of the Position

以增强UV-B(10.08 kJ.m-2.d-1)辐射后的小麦根尖细胞为材料,采用间接免疫荧光标记技术,利用激光共聚焦扫描显微镜,观察分析小麦根尖分裂期细胞Ran蛋白在分裂周期的分布及形态变化。研究结果显示,正常细胞中,Ran蛋白在细胞分裂间期主要定位于核膜周边,在后期定位于赤道板上和纺锤体上,末期又回到子细胞核膜周边;增强UV-B辐射处理后,在细胞分裂间期和前期有点状荧光分布在核膜的周围;中期和后期点状荧光分布在细胞质中;在末期部分点状荧光又回到核膜的周围,部分仍分散在核内,且出现落后染色体、染色体桥、不均等分裂等染色体畸变类型和异常分裂现象。

The distribution of Ran protein was examined by confocal laser scanning microscopy （CLSM） in the wheat root-tip cells under the enhanced ultraviolet-B （ 10. 08 kJ·m^-2·d^-1 ） radiation treated with indirect immunofluores-cence labeling technologies. The results showed that the normal cells in interphase, Ran was localized to the nucleus and the nuclear envelope as expected, at the equatorial plate and spindle during anaphase and at the surface of the daughter nuclei during telophase. After enhanced UV-B radiation treatment, there are some punctated fluorescence distribution in the nuclear envelope surrounding between mitotic interphase and prophase, in the metaphase and anaphase punctate fluo-rescence distribution in the cytoplasm; In telophase cells part of the pitting fluorscence returned to the nuclear enve-lope, some still disperse in the nucleus and some types of chromosome aberration were induced, including chromosomal logging, chromosomal bridge and partition-bundle division.