摘要
为研究亚硒酸钠(Na2SeO3)对游离脂肪酸(FFA)诱导大鼠肝细胞功能和Caspase 3活性变化的作保护用,采用胶原酶两步灌流法分离培养肝细胞,在细胞培养液中添加FFA和Na2SeO3(0.10μmol/L),测定肝细胞上清液生化指标、细胞增殖率和Caspase 3活性。结果表明,培养液中添加FFA,细胞增殖率降低,肝细胞功能受到影响,Caspase 3活性升高,且FFA的这种作用呈剂量依赖性;Na2SeO3能抑制由FFA引起的肝细胞的上述变化。
The experiment was conducted to study the effect of sodium selenite on liver function and the activity of Caspase 3 induced by free fatty acids （FFA）.Primary rat hepatocytes were obtained by two-step collagenase perfusion method.Cell proliferation rates,liver function and the activity of Caspase 3 in FFA-treated group with or without sodium selenite （0.10 μmol/L） were determined.The results showed that：FFA changed doseependently cell proliferation rates, liver function and the activity of Caspase 3, whereas the presence of sodium selenite reversed these alternations.
出处
《中国饲料》
北大核心
2013年第14期15-18,共4页
China Feed
基金
国家自然科学基金青年项目(31101868)
黑龙江省青年科学基金(QC2010057)
中国博士后科学基金特别资助(2012T50302)
东北农业大学大学生科创基金(2011056)
