牛磺酸对动脉粥样硬化兔NF-KB信号转导途径的影响

Effect of taurine on the NF-KB signal transduction pathway in atherosclerotic rabbits

目的观察牛磺酸对动脉粥样硬化(AS)兔IKBα、NF-KBp65、VCAM-1、hs-CRP表达的影响,探讨牛磺酸防治动脉粥样硬化的可能机制。方法将32只日本大耳白兔随机分为4组:正常对照组、AS模型组、牛磺酸治疗组、吡咯烷二硫代氨基甲酸盐(PDTC)治疗组,每组8只。8周后光学显微镜下观察主动脉病理形态学变化,Western blotting法检测IKBα、NF-KBp65蛋白表达,免疫组织化学染色SABC法检测VCAM-1表达,酶联免疫吸附法(ELISA)检测hs-CRP水平。结果①与正常对照组比较,AS模型组、牛磺酸治疗组、PDTC治疗组内膜厚度、泡沫细胞及斑块面积显著增加,尤以AS模型组最明显。②与AS模型组比较,牛磺酸治疗组与PDTC治疗组NF-KBp65、VCAM-1、hs-CRP显著下降(P<0.01),IKBα显著增加(P<0.01),牛磺酸治疗组与PDTC治疗组VCAM-1、hs-CRP比较差异无统计学意义,而IKBα与NF-KBp65的表达差异有统计学意义(P<0.05)。结论牛磺酸可能通过抑制NF-KB信号转导途径而下调炎症因子表达,从而抑制AS早期形成过程。

【Objectives】 To observe the effect of taurine on the level of serum hypersensitivity C-responsive protein（hs-CRP）,and the expressions of IKB α,NF-KBp65,VCAM-1 in the atherosclerotic rabbits,to explore its mechanism in the prevention and treatment of the atherosclerosis.【Methods】 Thirty-two male Japanese white rabbits were randomly divided into four groups： normal control group（n =8）,AS model group（n =8）,taurine group（n =8）,PDTC group（n =8）.All the rabbits were killed after eight weeks,and observed the pathological changes in aorta by optical microscope,the expression of IKB α,NF-KBp65 were detected by western blotting,the expression of VCAM-1 was detected by immunohistochemistry and the level of serum hs-CRP was detected by Enzyme-linked immunosorbent assay（ELISA）.【Results】 Compared with normal group,the intimal thickness,foam cell,plaque area of aorta were significantly increased in the other groups,especially in the AS model group.Compared with AS model group,the expression of NF-KBp65,VCAM-1,hs-CRP were significantly decreased in the taurine group and PDTC group（P ＆lt;0.01）,and the expression of IKB α were significantly increased in the taurine group and PDTC group（P ＆lt;0.01）.There were no difference about hs-CRP,VCAM-1,but the expression of IKB α and NF-KBp65 were different between them（P ＆lt;0.05）.【Conclusion】 Taurine play an important role in protection against atherosclerotic,which may be associated with reducing the expression of inflammation factors,such as hs-CRP,VCAM-1,by inhibiting the activity of NF-KB.