桂花品种群鉴定及其亲缘关系

Identification and Relationships of Osmanthus fragrans Cultivar Groups

利用3对叶绿体基因片段(psbJ-petA,psbA-trnH和trnS-G)和1对线粒体基因片段(nad7内含子2)对桂花品种群进行了初步研究,结果表明:4对基因片段都有一定的分辨率,其中trnS-G和nad7内含子2具有较多的信息位点,有潜力成为桂花种下等级鉴定的DNA条形码;桂花与其近缘种的分子系统树结果与经典分类基本一致,支持木犀属各组的划分;桂花品种起源很有可能存在种间或种内的基因交流,不是单物种起源,这或许与桂花雄全异株的繁育系统有关。

We used three informative chloroplast regions（psbJ-petA,psbA-trnH and trnS-G） and one mitochondrial region（nad7 intron2） for the identification and relationships of Osmanthus fragrans cultivar groups.Four pairs of genes are potential to become DNA barcoding of O.fragrans groups for their discriminabilities,especially trnS-G and nad7 intron 2.The phylogenetic results are mainly consistent with the classification of Osmanthus,in agreement with the section divisions of Osmanthus.There is gene exchange within O.fragrans groups or among Osmanthus rather than single origin of O.fragrans cultivars due to the androdioecy mating system of O.fragrans.