摘要
为了建立黄淮白山羊瘤胃甲烷菌快速检测的PCR方法,采集安徽本地黄淮白山羊瘤胃内容物,进行瘤胃甲烷菌的分离培养,利用瘤胃甲烷杆菌的保守基因甲基辅酶M还原酶基因(MCR)进行引物设计,并且优化PCR扩增条件。结果表明:所设计的引物具有特异性、专一性,优化的PCR扩增条件符合瘤胃甲烷杆菌扩增要求,扩增产物在140 bp的位置出现单一明亮条带。由此可见,利用PCR技术,可以快速、准确检测黄淮白山羊瘤胃甲烷杆菌。
An experiment was conduced to establish PCR technique to monitor methanogens of Huanghuai white goat.The rumen contents were collected from local Huanghuai white goat in Anhui to culture Methanobrevibacter ruminantium,and MCR gene sequences were amplified and optimized by PCR amplification.The primer is specific and the condition of amplification of PCR is suitable for reaction.A light bandofby the product appears at 140 bp.Therefore,PCR on M.ruminantium in rumen can accurately reflect the variation of rumen microorganism＇s population.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2013年第7期134-136,共3页
Journal of Northeast Forestry University
基金
安徽科技学院自然科学研究项目(ZRC2012310)
安徽科技学院大学生创新课题(12XSZ110)
关键词
PCR
瘤胃
甲烷菌
黄淮白山羊
PCR
Rumen
Methanobacterium ruminantium
Huanghuai white goat
