肉鸡小肠干细胞免疫组化定位和形态学研究

Study on the Distribution and Morphology of Broiler's Intestinal Stem Cells by Immunohistochemical Technology

观察肉鸡小肠各段肠干细胞的分布和形态结构特点,为探讨小肠干细胞与肠损伤修复之间的关系提供基础理论。采集处于生长阶段肉鸡(28日龄)的十二指肠、空肠、回肠,以Lgr5作为肠干细胞表记标记,运用免疫组织化学法研究肉鸡小肠各段中的肠干细胞分布和形态结构特点。结果表明,Lgr5作为肠干细胞表记标记特异性强,结果可靠。肠干细胞在肉鸡十二指肠、空肠、回肠均有分布,主要位于隐窝基底部潘氏细胞的+1～+4位置,细胞呈柱状或锥形,核椭圆形或染色不清。平均每个视野中肠干细胞数量十二指肠2个、回肠3个、空肠3个。本试验首次证实Lgr5可作为肉鸡肠干细胞的表面标记,并观察了肉鸡肠干细胞的形态结构,初步确定了肉鸡小肠中肠干细胞的数量,为进一步探讨肠干细胞在肉鸡生长发育中的作用奠定基础。

The study on the distribution and morphology of broiler’s intestinal stem cells by immunohistochemical technology had been carried on,which would explore the relationship between intestinal stem cells and intestinal injury.In this experiment,the duodenum,jejunum and ileum of the 28 day old broilers was collected.Lgr5 was selected to the intestinal stem cell marker and the distribution and morphology characteristics of broiler intestinal stem cell was observed by immunohistochemical method.The results showed that the specificity of Lgr5 as intestinal stem cells marker was strong.The intestinal stem cell was found in duodenum,ileum and jejunum of growing broiler,which were located in the ＋1 to the ＋4 position above paneth cells,and the cells were showed columnar or taperedconical,the nucleus were oval or blurring.The average number of intestinal stem cells in every microscope view was two in duodenum,three in ileum and three in jejunum.Therefore we confirmed that Lgr5 can be used as broiler intestinal stem cell markers,and the morphological structure of broiler intestinal stem cells was observed,the number of small intestine in the growing broiler was preliminarily determines,which will lay the foundation for further explore the role of intestinal stem cell in the growing broiler.