微波辅助提取枇杷叶黄酮类化合物工艺的优化

Optimization of microwave-assisted flavonoid extraction from loquat leaves

【目的】研究微波辅助法提取枇杷叶黄酮类化合物的最佳工艺条件,为进一步开发利用枇杷叶资源提供科学依据。【方法】以枇杷叶黄酮类化合物含量作为评价指标,探讨微波功率(W)、乙醇浓度(%)、料液比(g∶mL)和提取时间(s)对枇杷叶黄酮类化物提取率的影响,在单因素试验的基础上,利用正交试验筛选微波辅助提取枇杷叶黄酮类化物的最佳工艺条件。【结果】微波辅助乙醇提取枇杷叶黄酮类化合物的影响因素主次顺序为:乙醇深度>料液比>微波时间>微波功率,其最佳提取工艺条件为:乙醇浓度50%,微波功率125W,微波时间100s,料液比1∶10(g∶mL)。在最佳提取工艺条件下,得到黄酮类化物含量为0.712%。【结论】微波辅助乙醇提取枇杷叶黄酮类化合物得率高,且提取时间短、乙醇用量少,是提取枇杷叶黄酮类化合物的有效方法。

Objective The optimum technlogical conditions of microwave-assisted flavonoid extraction from loquat leaves were investigated to provide references for exploring and utilizing loquat leaves resources. Method With flavonoid content as assessment index, the influence of microwave power （W）, ethanol concentration（%）, solid-liquid ratio （g∶mL） and extraction time （s） on extraction efficiency of flavonoid was studied. The best parameters for microwave-assisted flavonoid extraction from loquat leaves were identified in orthogonal test on the basis of single factor experiment. ResultThe influencing sequence was ethanol concentration solid-liquid ratioextraction timemicrowave power. In the optimal parameters （ethanol concentration of 50%, microwave irradiated of 125 W, microwave time of 100 s and solid-liquid ratio of 1∶10（g∶mL））, the flavonoid content was 0.712%. Conclusion Extracting flavonoid from loquat leaves with the auxiliary of microwave is an effective method, owing to its high yield rate, brief extraction duration and less ethanol.