摘要
利用琼胶作唯一碳源,从腐烂的紫菜中分离筛选到1株高酶活力的琼胶降解细菌Z705。对其菌落形态、生理生化性质和酶活进行了初步研究,并克隆其16SrDNA序列,进行分子鉴定。结果表明:该菌株为革兰氏阴性,呈短杆状;与模式菌株嗜麦寡养食单胞菌Stenotrophomonas maltophilia(AB008509)的同源性为99%;根据表型特征、生理生化特性和分子鉴定将菌株Z705鉴定为寡养单胞菌属(Stenotrophomonassp.)。
Agar as the sole carbon source, to separation the screening plant height enzyme agarolytic bacteria Z705 from rotting seaweed. The colony morphology, physiological and biochemical properties and enzyme activity was studied, and the clone its 16 s rDNA sequence, molecular identification. The results showed that Z705 was gram negative, a short rod;strain Z705 displayed a high sequence similarity with Stenotrophomonas maltophilia （99%）. According to its morphological, physiological and biochemical properties and the homology analysis of 16S rDNA sequence, strain Z705 was identified as Stenotrophomonas sp.
出处
《中国农学通报》
CSCD
2013年第21期88-91,共4页
Chinese Agricultural Science Bulletin
基金
福建省科技重大专项"动物营养免疫调控技术集成及功能性饲料开发"(2010NZ0002)
关键词
琼胶酶产生菌
筛选
鉴定
寡养食单胞菌属
agar-decomposing bacteria
isolate
identification
Stenotrophomonas sp.
