摘要
在动物疫苗研制过程中,应用巢式PCR方法,设计两套引物,扩增支原体16S与23SrRNA基因间隔区序列,可以检测造成细胞污染的常见支原体种类。本试验应用该方法检测三批样品和阴性对照均无特异性目标条带,即三批疫苗样品支原体检测均为阴性。阳性对照样品在200～400bp之间出现特异性目标条带,实验表明所建立的巢式PCR方法是一种快捷、灵敏、准确的检测方法,可以用于检测动物疫苗细胞培养物中支原体污染。
In the exploration of animal vaccine, using nested-PCR method, we designed two pairs of primers, am- plified spacer region sequences between 16S and 23S rRNA gene of Mycoplasma. According to the result, we can detect common Mycoplasma species which cause cell contamination. By the application of the method, we detected of three batches of samples and negative controls, there was no specific target bands, showed the three batches of vaccine samples were negative for mycoplasma. There was a 200-400bp specific target band in the positive control sample. The method we established has fast, high sensitivity, good specificity, which can offer a technical reference for dection of Mycoplasma contamination in the vaccine culture.
出处
《中国动物保健》
2013年第7期21-23,共3页
China Animal Health
