截短侧耳素的萃取及结晶

Extraction and Crystallization of Pleuromutilin

研究截短侧耳素的萃取和结晶。截短侧耳素闪蒸粉分别用甲醇、乙醇和K酮在20℃、40℃和60℃条件下萃取,萃取时间60min,比较萃取率;60℃的K酮饱和溶液,分别降温至50℃、45℃和40℃,加入晶种保温3h,然后以5～8℃/h的速度降温至0℃,然后保温2h,离心获得晶体,50℃干燥1h,获得截短侧耳素粉,分析干燥失重、含量、杂质和收率。K酮在60℃时的萃取率最高,均值为92%;40℃加入晶种保温3h获得产品收率最高95.01%。K酮为更加理想的截短侧耳素萃取剂,萃取温度60℃,晶体成长的第一过程选择40℃。

Research extraction and crystallization of pleuromutilin. Pleuromutilin flash powder with methanol, ethanol and K ketone was extracted at 20 ℃, 40 ℃ and 60 ℃, extraction time 60 min, compared extraction rate. K ketone saturated solution at 60 ℃ was cooled to 50 ℃, 45 ℃ and 40℃, respectively, with addition of crystal warmed for 3h, and then cooled to 0 ℃ at the speed of 5-8℃ per 1 hour, then warmed for 2h, centrifugated for crystal, dryed for lh at 50 ℃, obtained pleuromutilin powder, loss on drying, content analysis, impurity and yield. K ketone extraction rate is the highest at 60 ℃, average is 92%. At 40℃ by adding crystal seeds obtained the highest yield products 95.01% for 3h. K ketone as truncated ear is more ideal element extractant, extraction tem- perature 60℃, crystal growth process at 40℃ first choice.