宽口径填充毛细管液相色谱柱柱尾结构的改进

Conical Column End for Wide Bore Packed Capillary Liquid Chromatographic Column

设计并评价了尾锥形填充毛细管液相色谱柱 ,与粘结型柱尾比较 ,前者柱效高且vanDeemter曲线高流速部分的流速 柱效关系明显改善。在折合流速为 1 0～ 1 5时 ,前者的柱效比后者高出 30 %～ 50 %。谱带的抛物线状的流形在尾锥形柱内得到改善 ,使色谱峰的对称性有明显提高。比较了两种结构的色谱柱在芳烃快速分析中的分离效果 ,结果是尾锥形柱的分离性能优于粘结型柱。

Wide bore (≥0 5 mm i d ) packed capillary liquid chromatographic columns with conical end were designed and evaluated It was found that the column efficiency was 30% 50% higher than that of the packed capillary columns with conventional end connection at optimal flow rate, and was twice as much as that at reduced flow rate of 10 15 The chromatographic peak symmetry and the van Deemter curve of the novel shape column were also improved substantially Fast analysis of PAHs is demonstrated