高效液相色谱法测定喘静片的3种主要成分

Determination of Three Principal Components in Chuanjing Tablets by Using High Performance Liquid Chromatography

采用高效液相色谱法同时测定喘静片中的 3种主要成分 :茶碱、异戊巴比妥和盐酸甲基麻黄碱。以ODS作固定相 ,通过均匀设计方法选择的最佳流动相为V (0 0 1 5mol/L磷酸二氢钠缓冲液 ,含体积分数为 0 3%的三乙胺 ,pH 4 9)∶V(甲醇 ) =35∶65,检测波长为 2 1 5nm ,用程序调节检测器的灵敏度 ,以咖啡因作内标物。茶碱、异戊巴比妥、盐酸甲基麻黄碱的回收率分别为 99 7%～ 1 0 2 6%,98 5%～ 1 0 0 2 %,98 0 %～ 1 0 2 7%,日间RSD分别为 0 2 3%～ 1 2 %,0 35%～ 2 5%,0 33%～ 1 6%。该方法简便、快速、准确。

Uniform design method was employed to optimize the mobile phase of HPLC in order to determine simultaneously three principal components, theophylline, amobarbital and methylephedrine hydrochloride, in Chuanjing tablet, a compound preparation for asthma and cough The stationary phase was ODS and the optimal mobile phase composition was V(0 015 mol/L phosphate buffer containning 0 3% triethylamine, pH 4 9)∶V(methanol)=35∶65 The detection was performed at 215 nm and the sensitivity was time programmed for simultaneous determination of minor and major components Caffeine was selected as the internal standard A baseline separation was achieved within 10 min The linear ranges for theophylline, amobarbital and methylephedrine hydrochloride were 0 03 g/L 0 20 g/L, \{7 5\} mg/L 50 0 mg/L and \{7 5\} mg/L 50 0 mg/L, recoveries 99 7% 102 6%, 98 5% 100 2% and 98 0% 102 7%, inter day RSDs 0 23% 1 2%, 0 35% 2 5%,0 33% 1 6% respectively This HPLC method is rapid and accurate, and suitable for the quality control of the preparation