反相高效液相色谱法测定罐头食品中乙二胺四乙酸的残留量

Determination of EDTA Residue in Canned Food Products by RP-HPLC

将罐头食品捣碎后用水稀释 ,加氯化铜溶液和抗坏血酸 ,用水定容 ,过 0 4 5μm滤膜后立即供液相色谱分析 ,在 2 54nm波长处测定。色谱柱 :HypersilODS(1 2 5mm× 4mmi.d .,5μm) ;流动相 :水 甲醇 (体积比为 80∶2 0 ) ,含有 2 0mmol/L四丁基溴化铵 ,0 0 3mol/L醋酸钠缓冲液 (pH 4) ,流速 :0 8mL/min ;进样量 :2 0 μL。ED TA在 1 0mg/L～ 40 0mg/L范围内呈线性。方法回收率为 95 5%～ 98 9%,其RSD为 0 82 %～ 1 32 %。

The homogenized sample is diluted with water After adding copper(Ⅱ) chloride solution and 20 mg of ascorbic acid, dilute the sample to the volume with water After thorough mixing and microfiltration over a 0 45 μm filter,the filtrate is ready for injection into the HPLC system and detected at 254 nm The 125 mm×4 mm i.d. chromatographic column is packed with Hypersil ODS 5 μm,eluted with a mobile phase of water methanol (80∶20,V/V) containing 20 mmol/L tetrabutyl ammonium bromide, 0 03 mol/L sodium acetate buffer (pH 4) at a flow rate of 0 8 mL/min. The injection volume is 20 μL. The response value was linear between 10 mg/L 400 mg/L The recovery was 95 5% 98 9% The RSD was 0 82% 1 32%