羊膜包裹对兔同种异体跟腱移植愈合的影响

Effect of human amnion package on allogenic achilles tendon transplantation healing in rabbits

目的探讨羊膜对兔同种异体跟腱移植愈合的影响及对粘连预防的作用。方法 24只新西兰白兔随机分为2组(n=12):实验组(羊膜组)在移植跟腱处包裹羊膜;对照组(无羊膜组)在移植跟腱处不包裹羊膜。术后观察动物一般情况,术后2、4周切取跟腱移植部位,常规制成电镜标本观察;于2、4、6周各组取4只动物行大体观察移植跟腱情况及力学检测。结果实验组跟腱周围均充血、水肿较对照组轻,肌腱细胞的内质网及其内容物较对照组多;跟腱最大拉伸断裂强度及最大滑动距离均大于对照组;且差异有统计学意义(P<0.05)。结论羊膜可以有效促进移植跟腱愈合及预防粘连。

Objective To investigate the effect of human amnion package on allogenic achilles tendon transplantation healing in rabbits.Methods Twenty-four New Zealand white rabbits were randomly divided into 2 groups.The 12 rabbits in experimental group were wrapped up with acellular amniotic membrane at the position of transplanted achilles tendon,however,the other 12 rabbits in control group were not wrapped up with acellular amniotic membrane at the position of transplanted achilles tendon.After the operation,the general condition of the rabbits was onserved,and 2,4 weeks after operation the tissues at the position of transplanted achilles tendon were taken to be made into specimens and to be observed by electron microscope,and at 2,4,6 weeks after operation the transplanted achilles tendon tissues of 4 rabbits in each group were taken to be generally observed and performed mechanics examination.Results At 2 and 4 weeks after operation,as compared with those in control group,the hyperemia and edema in experimental group were slighter,and there were a great deal of rough endoplasmic reticulum with abundant contents in tendon cells of experimental group;the maximum tensile strength and the sliding distance in experimental group were significantly greater than those in control group （P0.05） .Conclusion Human acellular amniotic membrane can promote effectively the healing of transplanted achilles tendon and can prevent adhesion.