胶束电动毛细管色谱法测定消糖灵中的优降糖

Separation and Determination of Glibenclamide in Xiaotangling Tablets by Micellar Electrokinetic Capillary Chromatography

建立了一种控制优降糖及其复方制剂质量的新方法。以甲氧苄氨嘧啶为内标 ,采用胶束电动毛细管色谱法分离测定了新药消糖灵中的优降糖。电泳条件 :以 2 5mmol/L硼砂 30mmol/L十二烷基硫酸钠 (pH 9 0 )为电泳介质 ,未涂层石英毛细管 (50 μmi.d .× 39 5cm ,有效分离长度 34 8cm)为分离通道 ,压力进样 (68 95kPa·s) ,1 7kV恒压电泳 (2 8℃ ) ,检测波长 2 2 8nm。优降糖在 1 4min内与其他成分得到很好分离 ,且质量浓度为 2 5mg/L～ 2 75mg/L时 ,优降糖可进行定量分析 ,加标回收率为 (1 0 0 6± 1 4 ) %。方法简便、快速 ,结果准确 ,重现性好 ,可用于优降糖复方制剂的质量控制。

A high performance capillary electrophoresis method has been developed for separation and quantitative analysis of glibenclamide in Xiaotangling tablets Electrophoretic conditions were as follows: micellar electrokinetic capillary chromatography (MECC) mode, trimethoprimum as internal standard,uncoated fused silica capillary (34 8 cm/39 5 cm(effective/total length),50 μm i d.),25 mmol/L borax 30 mmol/L SDS(pH 9 0), applied voltage 17 kV, (+)→(-), temperature 28 ℃, detection wavelength 228 nm, pressure injection 68 95 kPa·s Glibenclamide was separated successfully from other components within 14 minutes under the optimum conditions, and the relative peak area of glibenclamide increased linearly with the increase of its concentration within the range of 25 mg/L 275 mg/L The recovery was (100 6±1 4)% The method is simple, rapid and well reproducible, and can be used as a reliable tool for the quality control of Chinese traditional medicine containing glibenclamide