烟草中糖类物质的高效毛细管电泳-安培检测研究

Determination of Sugars in Tobacco by Capillary Electrophoresis with Amperometric Detection

将高效毛细管电泳－安培检测技术（ＨＰＣＥ－ＡＤ）用于不同烟草样品中糖类物质的测定。在 １×１０－６ｍｏｌ／Ｌ～１×１０－３ ｍｏｌ／Ｌ范围内，存在良好的线性关系，葡萄糖、果糖、蔗糖和麦牙糖的检测限均小于 ５．０ ×１０－７ｍｏｌ／Ｌ，结果令人满意。

High performance capillary electrophoresis with amperometric detection (HPCE/AD) was demonstrated for determining the different sugars in tobacco. Based on the good linearity between peak heihgt adn concentration of sugars, the quantities of different sugars were determined. The results showed that the method proposed was satisfactory in the analysis of tobacco.