摘要
报道了meso-四(4-甲基-3-磺酸苯基)卟啉[T(4-MP)PS4]作柱前衍生试剂生成铜、锌络合物,四乙基碘化胺(TEA·I)为离子对试剂。离子对高效液相色谱及光度检测快速分离测定铜、锌的方法。流动相为乙腈+水,内含乙酸.乙酸钠缓冲液和四乙基碘化胺。方法具有较高的灵敏度和选择性,并用于测定莲子和花生等食品中的痕量铜和锌。
A method based on ion-pair HPLC separation and photometric detection of Cu2+ and Zn2+ by their complexes of meso-tertra (4-methyl-3-sulfophenyl-porphyrin [T(4- MP) PS4 ] has been presented. They are separated on an ODS column within 11 min.Using eluant composed of acetonitrile-water (38.0%) solution containing 50 mmol/L tetrathylammonium iodide and 10 mmol/L acetic acetic acid-sodium acetate buffer(pH 4.1) and detected at 420 nm. Trace amount of Zn and Cu in Peanut and lotus seed have been successfully determined by this method.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2000年第10期1260-1262,共3页
Chinese Journal of Analytical Chemistry
基金
浙江省自然科学基金资助项目
关键词
HPLC
分离
卟啉
光度法
络合物
铜
锌
痕量分析
Ion-Pair high performance liquid chromatography
meso-tetra (4 - methyl - 3 - sulfophenyl ) porphyrin
copper
zinc