离子对高效液相色谱分离及光度检测法测定meso-四(4-甲基-3-磷酸苯基)卟啉-铜、锌络合物

Tetra(4-methyl-3-sulfophenyl) -porphyrin Complexes by Ion-pair High Performance Liquid Chromatography and Photometric Detection

报道了ｍｅｓｏ－四（４－甲基－３－磺酸苯基）卟啉［Ｔ（４－ＭＰ）ＰＳ４］作柱前衍生试剂生成铜、锌络合物，四乙基碘化胺（ＴＥＡ·Ｉ）为离子对试剂。离子对高效液相色谱及光度检测快速分离测定铜、锌的方法。流动相为乙腈＋水，内含乙酸．乙酸钠缓冲液和四乙基碘化胺。方法具有较高的灵敏度和选择性，并用于测定莲子和花生等食品中的痕量铜和锌。

A method based on ion-pair HPLC separation and photometric detection of Cu2+ and Zn2+ by their complexes of meso-tertra (4-methyl-3-sulfophenyl-porphyrin [T(4- MP) PS4 ] has been presented. They are separated on an ODS column within 11 min.Using eluant composed of acetonitrile-water (38.0%) solution containing 50 mmol/L tetrathylammonium iodide and 10 mmol/L acetic acetic acid-sodium acetate buffer(pH 4.1) and detected at 420 nm. Trace amount of Zn and Cu in Peanut and lotus seed have been successfully determined by this method.