生防菌混合培养液对烟草普通花叶病毒的抑制作用

Disinfection Effect of Antagonistic Bacteria Cultures on Tobacco Mosaic Virus

由烟草普通花叶病毒（Tobacco mosaic virus,TMV）引起的病毒病是烟草上发病早、传播快的主要病害之一,农事操作机械接触传染及土壤病残传毒是TMV的主要流行因子。为应用生防菌进行土壤和工具消毒,对已筛选的5株生防菌（Ba、CZ、A3、4A1、2A2）,将其单菌株培养液及菌株两两混合接种后的培养液与TMV等体积混合,采用摩擦接种枯斑寄主三生NN烟的侵染力测定法进行菌株活性比较。结果显示,5株生防菌培养液及两两混合接种后的培养液对TMV均有较强的抑制活性,与NB培养基对照相比,其5倍稀释液对TMV的抑制效果为94%~99%,以CZ＋2A2混合接种培养液对TMV抑制效果最好,50倍稀释液防效为74.9%。具备单独或混合培养制备土壤消毒剂用以工具及苗床和大田土壤消毒的潜力。

Tobacco mosaic virus （TMV） is one of the major diseases on tobacco, spreading fast and popular early. Mechanical contact infection in farm work and transmission of virus residues in soils are the major culprits for the rampant outbreak of TMV. For application of bio-control bacteria in soil and instruments disinfection, the suppression in vitro of the five single bacteria cultures and the twain bacteria cultures on TMV was investigated on local lesion host of Nicotiana tabacum var. samsum NN challenged by mechanical inoculation of mixture of cultures with TMV. The results showed that both of the five single bacteria cultures and the twain bacteria cultures had good disinfection effect on TMV, all of 5-fold dilution cultures suppressed TMV from 94% to 99% in vitro compared to the mixture of NB with TMV. And the twain bacteria cultures of CZ and 2A2 had the best disinfection effect, with 74.90% of suppression in vitro by 50-fold dilution cultures. These results indicated that CZ and 2A2 has a potential to be an instruments and soils disinfector against TMV.