LXA4及其受体在子痫前期中的表达及其意义

EXPRESSION AND CORRELATION OF HUMAN LIPOXIN A4 AND ITS RECEPTOR IN PREECLAMPSIA

目的:探讨子痫前期患者血清中脂氧素A4(Lipoxin A4,LXA4)及胎盘组织中脂氧素A4受体(Lipoxin A4receptor,ALX-R)的表达及其临床意义。方法:采用ELISA方法检测子痫前期组与对照组孕妇血清中LXA4的含量;采用免疫组化方法、RT-PCR方法检测子痫前期组与对照组孕妇胎盘组织中ALX-R mRNA及蛋白的表达情况。结果:(1)ELISA方法显示子痫前期组与对照组均有LXA4的表达,轻度子痫前期组与重度子痫前期组、对照组比较血清中LXA4的含量升高,差异有统计学意义(P<0.05),而重度子痫前期组血清LXA4的含量与对照组比较差异无统计学意义(P>0.05);(2)胎盘组织ALX-RmRNA的表达随着病情的严重程度而明显下降,3组之间比较,差异有统计学意义(P<0.01);(3)ALX-R的蛋白表达随着病情的严重程度而下降,3组之间比较,差异有统计学意义(P<0.01)。结论:LXA4、ALX-R的表达可能与子痫前期发生发展的病理生理过程密切相关。

Objective：The purpose of this study was to investigate the expression and its significance of an antiinflammatory lipid mediator, lipoxin A4 （LXA4）, and lipoxin A4 receptor （ALX-R） in placenta of pa- tients with preeclampsia. Methods： ELISA was used to detect the expression of LXA4 in blood serum, im- munohistochemistry and PCR were used to detect the expression of protein and mRNA for ALX-R in the placenta of 30 women with preeclampsia （including 10 cases of mild preeclampsia, and 20 cases of severe preeclampsia） and 20 normal full-term pregnant women, respectively. Results： （1） Levels of LXA4 in women with mild preeclampsia were high （ P〈0.05）. There was no difference between normal pregnancy and severe preeelampsia （ P〉0.05）. （2） The mRNA expressions of ALX-R was lower in women with preeclampsia than in control women （ P〈0.01）, much less the expression has decreasing tendency with aggravation; （3） The expression of ALX-R protein was lower in women with preeclampsia than in control group （ P〈0.01）. Conclusion： The abnormal expression of LXA4 and ALX-R in placenta may play an important role in the pathogenesis of preeclampsia.