黔产红花石蒜中生物碱成分的分离与鉴定

Isolation and identification of alkaloids of bulbs of Lycoris radiata Herb. produced in Guizhou province

目的对黔产红花石蒜的化学成分进行分离与鉴定。方法应用硅胶柱色谱、葡聚糖凝胶柱色谱和半制备高效液相色谱分离纯化,根据理化性质和波谱数据确定化合物的结构。结果从黔产红花石蒜中分离得到11个化合物,分别鉴定为加兰他敏(galanthamine,1)、水仙花碱(tazettine,2)、网球花胺(haemanthamine,3)、去甲基加兰他敏(O-demethylgalantamine,4)、2-羟基-6-甲氧基香水仙灵(2α-hydroxyl-6-O-methyloduline,5)、小星蒜碱(hippeastrine,6)、高石蒜碱(homolycorine,7)、9-O-去甲基高石蒜碱(9-O-demethylhomolycorine,8)、葱莲碱(zephyranthine,9)、力可拉敏(lycora-mine,10)、6α-去氧-8-氧多花水仙碱(macronine,11)。结论化合物3、5和11为首次从该属植物中分离得到,化合物2和4为首次从该种植物中分离得到,同时首次对化合物11进行了NMR数据的归属。

Objective To study the alkaloids from the bulbs of Lycoris radiata.Herb.of Guizhou province.Methods The alkaloids were isolated by chromatographic methods such as silica gel,ODS,Sephadex LH-20 and HPLC,and identified by the chemical and physical methods,especially spectral analysis.Results Eleven alkaloids were isolated.The structures of them were identified as galanthamine（1）,tazettine（2）,haemanthamine（3）,O-demethylgalantamine（4）,2α-hydroxyl-6-O-methyloduline（5）,hippeastrine（6）,homolycorine（7）,9-O-demethylhomolycorine（8）,zephyranthine（9）,lycoramine（10）,macronine（11）.Conclusions Compounds 3,5 and 11 are obtained from the genus Lycoris,and compounds 2 and 4 are obtained from L.radiata Herb.for the first time.The NMR data of compound 11 is assigned for the first time.