药用大黄中蒽醌和非蒽醌类成分的分离与鉴定

The anthraquinone and non anthraquinone constituent of Rheum officinale Baill.

目的对药用大黄中蒽醌及非蒽醌类成分进行深入研究。方法药用大黄80%(φ)乙醇溶液提取物经硅胶柱、ODS柱、MCI gel CHP20、SephadexLH-20凝胶柱色谱及HPLC色谱分离,纯化,根据理化性质和波谱数据进行结构鉴定。结果分离得到6个蒽醌类和5个非蒽醌类成分,经波谱数据解析,分别鉴定为香兰基丙酮(vanillylacetone,1)、大黄素(emodin,2)、芦荟大黄素(aloeemodin,3)、大黄素甲醚-8-O-β-D-葡萄糖苷(physcion-8-O-β-D-glucopyranoside,4)、大黄酚-8-O-β-D-葡萄糖苷(chrysophanol-8-O-β-D-glucopyranoside,5)、1-甲基-8-羟基-9,10-蒽醌-3-O-β-D-(6'-O-桂皮酰基)吡喃葡萄糖苷(1-methyl-8-hydroxyl-9,10-anthraquinone-3-O-β-D-(6'-O-cinnamoyl)glucopyrano-side,6)、芦荟大黄素-3-(羟甲基)-O-β-D-葡萄糖苷(aloe emodin-3-(hydroxymethyl)-O-β-D-glucopy-ranoside,7)、(+)儿茶素(catechin,8)、表儿茶素没食子酸酯(epicatechin-3-O-gallate,9)、儿茶素-3-O-β-D-葡萄糖苷(catechin-3-O-β-D-glucopyranoside,10)、儿茶素-8-β-D-葡萄糖苷(catechin-8-β-D-glucopyranoside,11)。结论化合物1,6,9-11为首次从该植物中分离。

Objective To study the anthraquinone and non anthraquinone constituents of Rheum officinale Baill..Methods 80% Ethanol extraction of Rheum officinale Baill.was isolated and purified by silica gel,ODS,MCI gel CHP20,SephadexLHK-20 gel,HPLC and their structures were elucidated on the basis of NMR data and physico-chemical property.Results six anthraquinone and five non anthraquinone constituents were isolated and their structures were identified as vanillylacetone（1）,emodin（2）,aloe emodin（3）,physcion-8-O-β-D-glucopyranoside（4）,chrysophanol-8-O-β-D-glucopyranoside（5）,1-methyl-8-hydroxyl-9,10-anthraquinone-3-O-β-D-（6′-O-cinnamoyl）glucopyranoside（6）,aloeemodin-3-（hydroxymethyl）-O-β-D-glucopyranoside（7）,（＋）catechin（8）,epicatechin-3-O-gallate（9）,catechin-3-O-β-D-glucopyranoside（10）,catechin-8-β-D-glucopyranoside（11）.Conclusions The compounds 1,6,9-11 are isolated from rhubarb for the first time.