微生物絮凝剂产生菌XN-5的筛选及其絮凝活性试验研究

Screening of Strain XN-5 That Produces Microbial Flocculant and the Test on Its Flocculation Activity

为了解决无机和高分子絮凝剂处理的水体对人体有害、且产生二次污染等问题,本文采用富集培养、稀释平板分离法从活性污泥中筛选出一株具有稳定、高絮凝活性的细菌XN-5作为生物絮凝剂菌株,并通过试验研究了该菌株在不同培养时间、初始pH值、装液量和絮凝剂投入量条件下的絮凝率以及絮凝活性分布。结果表明:通过生化显色反应初步确定该菌株产生的絮凝剂主要是多糖类胞外产物;在初始pH值为7、絮凝剂投入量为2mL/100mL高岭土悬浊液、培养时间为48h、装液量为50mL/250mL三角瓶的最佳培养条件下,该菌株产生的絮凝剂的絮凝率达到了93.1%。

For the purpose o lant is harmful to human bo culture and dilution plate to f solvin dy and screen g the problem that the wat causes secondary pollution a strain XN5 with stable sludge as microbial flocculating strain. The paper experimenta ring activity distribution of the strain under different kinds pacity and the amount of inputs. The results show that the f the extracellular product of polysaccharide, which is prelimi the flocculation rate reaches 93.1G under the optimal cultu of flocculant 2/100 mL,temperature 30℃ ,cultural time 48h er treated by inorganic ,this paper adop and high floccul lly studies the fl ts the and polymeric floccu method of enrichment ation activity from activated occulation rate and floccula of culture time, initial locculant produced by pH,culture medium ca the flocculating strain is narily determined by biological colar reaction re conditions when initial pH is 7,the amount and culture medium capacity 50/250 mL.