液相色谱紫外法测定鸡肉中三种硝基咪唑残留量

DETERMINATION OF THREE KINDS OF NTTROIMIDAZOLE RESIDUES EN CHICKEN MUSCLE BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

建立了测定鸡肉中二甲硝咪唑、罗硝唑、甲硝唑残留量的方法。以二氯甲烷提取,硅胶固相萃取净化,液相色谱紫外法检测。样品添加10ug/kg,50ug/kg,平均回收率为78％～91％,相对标准偏差为6.4％～10.1％。

A HPLC method for determining metronidazole(MTZ),ronidazole (RNZ) and dimetridazole (DMZ) in chicken muscle was developed.Nitroimidazole compounds were extracted with dichloromethane,followed by solid phase extraction cleanup using silica gel cartridges.Nitroimidazole were eluated with acetone and the eluants were evaporated to dryness at 40℃under nitrogen.The residue was redissolved in the moblie phase.HPLC separation was done on an ODS column with ACN/0.05 mol/L ammonium acetate buffer( pH 4.5) (1:6,V/V) as mobile phase.Ultraviolet detection was performed at 320 nm.The mean recoveries of nitroimidazoles spiked at 0.01 mg/kg and 0.05 mg/kg was MTZ 85%,RNZ 89%,DMZ 80%,with RSDs of 6.8%,7.8% and 9.3%.