北豆根总生物碱纯化工艺研究

Study on Purification Technology of Total Alkaloids from Rhizoma Menispermi

目的:优化北豆根总生物碱纯化工艺条件。方法:采用紫外分光光度法测定北豆根总生物碱含量,优选大孔吸附树脂最佳纯化分离工艺。结果:AB-8型大孔吸附树脂柱对北豆根总生物碱最大吸附量为0.7g/g(药材量∶树脂湿重),平均洗脱率66.56%,平均总生物碱转移率为63.49%,最终产品的生物碱纯度达到70.79%。结论:AB-8型大孔吸附树脂可用于纯化北豆根总生物碱,该方法简便可行。

Objective： To optimize the purification technology of total alkaloids in rhizoma menispermi. Methods： Using the content of total alkaloids as the index determined by ultraviolet spectrophotometric,and the optimum purification process was determined by resin. Results： Each gram of AB-8 resin could absorb 0. 7 g/g of the total alkaloids,and the desorption ratio was 66. 56% . The transfer rate of total alkaloids was 63. 49% . The product purity of total alkaloids was 70. 79% . Conclusion： The AB-8 resin can be used for purificating total alkaloids from rhizoma menispermi and the established procedure is simple and feasible.