植物药-茯苓菌双向发酵代谢特征初探

Primary exploration of metabolic characteristics in Poria cocos fermentation system with adding botanical medicine factors

目的对植物药-茯苓菌共发酵体系进行初步研究。方法通过菌株筛选、碳源初筛、氮源初筛等确定茯苓菌发酵培养基,并将12种常见植物药粉末添加到茯苓菌发酵体系,观察其对茯苓胞外多糖合成和菌丝体生长的影响。结果茯苓菌ZK表现出较强的胞外多糖合成能力和细胞生长能力,2%葡萄糖+1%玉米粉为最佳碳源,0.5%酵母膏为最佳氮源;黄芪、茶叶、麦芽、三七、葛根、穿心莲表现出极强的胞外多糖合成促进能力;黄芪、茶叶、穿心莲、鹿衔草可显著促进茯苓菌丝体的生长,鹿衔草却抑制茯苓胞外多糖的合成或分泌,枸杞、山楂、丹参、茜草、连翘则抑制茯苓菌丝的生长。结论植物药因子对茯苓菌的胞外多糖合成和菌丝体生长具有明显的调控作用。

Objective To study the metabolic characteristics of Poria cocos fermentation system.Methods Using the biomass yield and exopolysaccharide concentration as the main evaluation indices to choose the excellent Poria cocos strain and to determine the best carbon source and the best nitrogen source for Poria cocos fermentation.The effects of 12 botanical drug factors on the biosynthesis of Poria cocos exopolysaccharides and the growth of Poria cocos were detected by adding to Poria cocos fermentation system.Results Poria cocos ZK had more effects on promoting exopolysaccharides biosynthesis and Poria cocos growth.The best carbon source was 2% glucose mixed with 1% corn starch and the best nitrogen source was 0.5% yeast extract.During the 12 chosen botanical medicine factors,Huangqi,Tea-leaves,Malt,Sanqi,Gegen and Chuanxinlian showed excellent promotion for Poria cocos exopolysaccharides biosynthesis,while Huangqi,Tea-leaves,Chuanxinlian and Luxiancao showed remarkable increase in Poria cocos growth.However,Luxiancao inhibited Poria cocos exopolysaccharides biosynthesis or secretion,and Gouqi,Shanzha,Danshen,Qiancao,Lianqiao obviously suppressed Poria cocos growth.Conclusion Botanical medicine factors have certain regulating effects on Poria cocos exopolysaccharides biosynthesis and Poria cocos cell growth.