期刊文献+

寡核苷酸自动合成试剂的研究进展 被引量:2

Progress on automated oligonucleotide synthesis reagents
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摘要 主要介绍近几年来核酸自动化固相合成试剂的研究进展。 This review is focused on the late progress of the reagents for automated oligonucleotide synthesis.
出处 《化学试剂》 CAS CSCD 2000年第5期285-288,284,共5页 Chemical Reagents
基金 国家"863"!(编号 10 2 - 0 8- 0 4 - 0 1) 军队"九五"重点课题!(编号 962 0 0 0 7)资助
关键词 寡核苷酸 固相合成 保护基 活化剂 化学合成 oligonucleotide solid phase synthesis protecting group activator support oligonucleotide analogue
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参考文献5

  • 1Agrawal S,Antisense Nucleic Acid Drug Dev,1998年,8卷,135页
  • 2Ma M,Nucleic Acids Res,1997年,25卷,3590页
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同被引文献29

  • 1伍方勇.寡核苷酸分离纯化新技术[J].生命科学仪器,2004,2(4):53-54. 被引量:2
  • 2王升启,马立人.高效薄层色谱法纯化合成寡核苷酸及其衍生物[J].生物化学与生物物理进展,1993,20(3):234-237. 被引量:7
  • 3任吉存,黎众魁,夏林庆,姚开泰.毛细管凝胶电泳分离和检测低聚核苷酸和脱氧核糖核酸的合成产物[J].分析化学,1996,24(3):330-332. 被引量:4
  • 4Kumar RK, Olsen P, Ravikumar VT. An alternative advantageous protocol for efficient synthesis of phosphorothioate oligonucleotides utilizing phenylacetyl disulfide (PADS) [ J ]. Nucleosides Nucleotides Nucleic Acids, 2007, 26(2) : 181 -188.
  • 5Krotz AH, Gorman D, Mataruse P, et al. Phosphorothioate oligonucleotides with low phosphate diester content: greater than 99. 9% sulfurization efficiency with "aged" solutions of phenylacetyl disulfide (PADS) [ J ]. Org Process Res Dev, 2004, 8 (6) : 852 -858.
  • 6Cheruvallath ZS, Kumar RK, Rentel C, et al. Solid phase synthesis of phosphorothioate oligonucleotides utilizing diethyldithio-carbonate disulfide (DDD) as an efficient sulfur transfer reagent [J]. Nucleosides Nucleotides Nucleic Acids, 2003, 22 (4): 461 -468.
  • 7Krotz AH, Carry RL, Moore MN, et al. Synthesis of antisense oligonucleotides using environmentally friendly and safe deprotection procedures[J]. Green Chem, 1999, 1 : 277 -282.
  • 8Deshmukh RR, Warner TN, Hutchison F, et al. Large-scale purification of antisense oligonucleotides by high-performance membrane adsorber chromatography[ J]. J Chromatogr A, 2000, 890(1) : 179 -192.
  • 9Arghavani MB, Romano LJ. A method for the purification of oligonucleotides containing strong intraor intermolecular interactions by reversed-phase high-performance liquid chromatography [J]. Anal Biochem, 1995, 231(1) : 201 -209.
  • 10Fearon KL, Hirschbein BL, Nelson JS, et al. An improved synthesis of oligodeoxynucleotide N3'-> P5' phosphoramidates and their chimera using hindered phosphoramidite monomers and a novel handle for reverse phase purification [ J ]. Nucleic Acids Res, 1998, 26(16) : 3813 -3824.

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