新疆阿克苏盐碱地土壤细菌的分离研究

Isolation of Bacteria in the Saline Alkaline Soils of Akesu in Xinjiang

为从可培养层面了解新疆塔里木盆地盐碱地土壤中的细菌资源多样性,本研究共分离和培养盐碱地土壤中的细菌103株。16S rDNA基因序列系统发育分析表明,这些株菌分别属于芽胞杆菌属(Bacillus)等31个属。盐碱地土壤环境中的优势菌群是Bacillus,Streptomyces,Micrococcus,Kocuria和Paracoccus,分别占总菌株数的36.27%、10.8%、6.9%、6.9%和3.9%。其中5株菌的16S rDNA基因序列与已有效发表菌株的序列相似性小于97%,表明它们可能是新的细菌分类单元。研究表明新疆阿克苏地区盐碱地土壤中具有丰富的微生物资源,为盐碱地微生物资源的开发提供重要的理论依据。

In order to understand the diversity of microbial resources in culture media from the saline soil of Tarim Basin in Xinjiang, we isolated and cultured 103 strains of the bacteria from the saline alkaline soils of Akesu in Xinjiang. 16S rDNA sequence phylogenetie analysis showed that these strains belong to the genus Bacillus and other 31 genera. The dominant strains were Bacillus, Streptomyces, Micrococcus, Kocuria and Paracoccus ,which occupied 36.27% , 10.8% , 6.9%, 6.9% and 3.9% of the total strains. 16S rDNA of five strains had less than 97% sequence similarity to those published strains, suggesting that they might be new bacterial taxa. The results showed that there were abundant micriobial resources in the saline alkaline soils of Aksu in Xinjiang, providing an important base for the development of microbial resources in saline alkaline soils.