扩张型心肌病伴心力衰竭患者肝细胞生长因子与心功能的相关性研究

Relationship between content of hypatocyte growth factor and heart function in patients with dilated cardiomyopathy accompanied by heart failure

目的探讨扩张型心肌病伴心力衰竭患者血清肝细胞生长因子(HGF)浓度与心功能分级的关系。方法纳入2011年至2013年扩张型心肌病伴心力衰竭患者63例作实验组,进一步按照NYHA心功能分级分为Ⅰ级组(n=15)、Ⅱ级组(n=16)、Ⅲ级组(n=15)和Ⅳ级组(n=17),纳入同期健康志愿者32例作对照组。采用酶联免疫吸附法(ELISA)测定HGF浓度,电化学发光法测定血清N端脑钠肽前体(NT-proBNP)浓度,行心脏超声检查左心室舒张末期内径(LVEDD)和左心室射血分数(LVEF),并采用线性回归分析血清HGF浓度和上述指标的相关性。结果实验组各亚组的HGF浓度、NT-proBNP浓度、LVEDD值均高于对照组,差异有统计学意义(P<0.05),且心力衰竭程度越重,上述指标值越高,各组两两之间均存在统计学差异(P<0.05);实验组各亚组LVEF值均低于对照组,差异有统计学意义(P<0.05);血清HGF浓度与NT-proBNP浓度和LVEDD均呈正相关,相关系数分别为0.79和0.73,与LVEF值负相关,相关系数为-0.69(P均<0.05)。结论扩张型心肌病伴心功能不全患者血清HGF浓度和心功能不全的严重程度相关,对判断心功能不全严重程度有帮助。

Objective To investigate the relationship between content of hypatocyte growth factor （HGF） and grading of heart function in the patients with dilated cardiomyopathy accompanied by heart failure. Methods The patients （n=63）were chosen as experiment group from 2011 to 2013, and further divided, according to grading of heart function, into grade Ⅰ group （n=15）, grade Ⅱ group （n=16）, grade Ⅲ group （n=lS） and grade Ⅳ group （n=17）. At the same time 32 healthy volunteers were chosen as control group. The level of serum HGF was detected by using ELISA, and the level of serum NT-proBNP was detected by using electrochemiluminescence immunoassay （ECLIA）. The changes of LVEDD and LVEF were detected by using cardiac ultrasonography. The relationship between the level of serum HGF and above indexes was analyzed by applying linear regression. Results The levels of serum HGF and NT-proBNP and LVEDD were higher in all subgroups of experiment group than those in control group （P〈0.05） and increased as exacerbating of heart failure with statistical differences between any different 2 groups. LVEF was lower in all subgroups of experiment group than that in control group （P〈0.05） and was more significant as exacerbating of heart failure. The levels of serum HGF and NT-proBNP were positively correlated to LVEDD （correlation coefficients： 0.79 and 0.73）, and were negatively correlated to LVEF （correlation coefficient： -0.69,P〈0.05）. Conclusion The level of HGF was correlated to the severity of heart failure in the patients with dilated cardiomyopathy accompanied by heart failure, which was related with the the severity of heart failure.