金属离子对1,6-二磷酸果糖合成代谢流量的影响

Influence of Metal Ion on Metabolic Pathway Flux of Fructose-1,6-diphosphate Synthesis

本文首次建立了1,6-二磷酸果糖(FDP)合成代谢途径流量模型，并应用该模型求解FDP合成过程中代谢流量分配。研究了NH4+、K+、Mg2+对1,6-二磷酸果糖合成代谢途径流量的分配的影响，结果发现NH4+、K+、Mg2+对代谢途径流量分配均有一定的影响；尤其是在这三者共存的情况下，代谢流量分配发生了重大改变，1,6-二磷酸果糖的累积流量达到41.9，为化学调节1,6-二磷酸果糖的超量生产提供了理论基础。

A model of metabolic pathway flux of fructose-1,6-diphosphate (FDP) accumulation has been proposed in this paper. Using this model, the metabolic flux distribution in FDP accumulation process was solved. The influences of NH4+, K+ and Mg2+ on the distribution of metabolic pathway flux of FDP were studied. The results showed that these metal ions had affected the metabolic pathway flux of FDP on different degree. The distribution of metabolic pathway flux was changed greatly when NH4+, K+ and Mg2+ were added together, and the flux of accumulation of FDP was up to 41.9 from 16.1. This provided the theoretical basis for overproduction of FDP.