初诊鼻咽癌患者外周血CD4^+CD25^+Foxp3^+调节性T细胞的变化及与TGF-β1的相关性

The Changes of CD4^+CD25^+Foxp3^+ Regulatory T Cell in Untreated Nasopharyngeal Carcinoma Patients of Peripheral Blood and the Correlativity with TGF-β1

目的:观察初诊鼻咽癌患者外周血CD4+CD25+Foxp3+调节性T细胞的变化及与TGF-β1的相关性。方法:采用流式细胞术检测30例正常对照者(对照组)及47例初诊鼻咽癌患者(患者组,临床TNM分期Ⅰ期11例、Ⅱ期16例、Ⅲ期12例、Ⅳ期8例)外周血单个核细胞CD4+CD25+Foxp3+调节性T细胞数目,并计算CD4+CD25+Foxp3+调节性T细胞占CD4+T淋巴细胞的百分比;酶联免疫吸附试验法(ELISA)检测其血清TGF-β1水平,分析CD4+CD25+Foxp3+调节性T细胞与TGF-β1的相关性。结果:与正常对照组比,患者组PB-MC CD4+CD25+Foxp3+Treg占CD4+T淋巴细胞的百分比、CD4+CD25+Foxp3+Treg绝对数及血清TGF-β1水平均显著升高,差异有显著的统计学意义(P<0.01,P<0.05)。相关性分析表明,CD4+CD25+Foxp3+Treg/CD4+T及CD4+CD25+Foxp3+Treg绝对数均与血清TGF-β1水平呈明显正相关(r分别为0.737、0.679,P<0.01)。结论:初诊鼻咽癌患者外周血TGF-β1及CD4+CD25+Foxp3+Treg增高,TGF-β1可能参与诱导CD4+CD25+Foxp3+Treg的生成,与CD4+CD25+Foxp3+Treg相互作用,从而发挥免疫抑制作用。

Objective To observe the changes of CD4＋CD25＋Foxp3＋ regulatory T cell in untreated nasopharyngeal carcinoma patients of peripheral blood and the relationship between CD4＋ CD25＋ Foxp3＋ regulatory T cell with the TGF-β1.Methods The CD4 ＋ CD25 ＋ Foxp3 ＋ regulatory T cell of peripheral blood in 47 patients with untreated nasopharyngeal carcinoma（TNMⅠ11,Ⅱ16,Ⅲ 12,Ⅳ8） and 30 normal healthy persons served as control group.All of patients and controls was detected by flow-cytometry.The level of serum TGF-β1 was detected by ELISA.The relationship between the proportion and number of CD4 ＋ CD25 ＋ Foxp3 ＋ regulatory T cell with TGF-β1 were statistically analyzed.Results Compared with control group,the proportion and number of CD4 ＋ CD25 ＋ Foxp3 ＋ regulatory T cell and TGF-β1 in nasopharyngeal carcinoma patients of peripheral blood were increased significantly（P 0.01,P 0.05）.The proportion and number of CD4 ＋ CD25 ＋ Foxp3 ＋ regulatory T cell were postive correlated with the level of serum TGF-β1.Conclusion The level of TGF-β1,the proportion and number of CD4 ＋ CD25 ＋ Foxp3 ＋ regulatory T cell were increased in untreated nasopharyngeal carcinoma patients of peripheral blood.TGF-β1 may play an important role in generation of CD4 ＋ CD25 ＋ Foxp3 ＋ regulatory T,which interacting with CD4 ＋ CD25 ＋ Foxp3 ＋ regulatory T and in result to exert of immunosuppression.