期刊文献+

银杏叶提取物预处理对红藻氨酸致痫大鼠神经肽Y表达的影响 被引量:3

Influence of Ginkgo biloba extract pretreatment on neuropeptide Y expression of kainic acid induced epileptic rats
下载PDF
导出
摘要 目的观察银杏叶提取物(GBE)预处理给药对癫痫大鼠的干预效应及其与神经肽Y(NPY)表达的关系,以探讨GBE的抗癫痫机制。方法雄性SD大鼠侧脑室注射红藻氨酸(KA)建立癫痫动物模型,将72只SD大鼠随机均分为假手术组(A组)、GBE对照组(B组)、癫痫模型组(C组)、GBE预处理组(D组),各组再分为造模成功后6、12、24 h处死这3个亚组,每个亚组6只大鼠。采用HE染色观察海马组织中神经元细胞形态变化,用免疫组织化学技术测定海马组织中NPY蛋白的表达。结果 D组大鼠癫痫发作级别及神经元损害较C组降低;C组3个亚组的大鼠海马组织中NPY蛋白表达较A组相对应的各亚组明显增多(P<0.05);D组中3个亚组大鼠海马组织中NPY蛋白的表达较C组相对应的各亚组明显增多(P<0.05);B组与C组相比相关指标均无统计学意义;同组内6、12、24 h 3个时间点NPY蛋白表达呈上升趋势。结论 GBE预处理给药有一定的抗癫痫作用,其抗癫痫的机制与减轻神经元的损害、减轻癫痫发作程度及NPY蛋白表达上调有关。 Objective To investigate the intervention effects of Ginkgo biloba extract (GBE) pretreatment on epi- leptic rats and its relationship with the expression of neuropeptide Y ( NPY), and to probe the anti-epileptic mecha- nism of GBE. Methods An animal model of complex partial epilepsy was established in male SD rats by intracere- broventricular injection of kainic acid. 72 SD rats were randomly divided into sham operation group ( group A), GBE control group (group B), epilepsy model group (group C), and GBE pretreatment group (group D). Each group was further divided into three subgroups (6, 12 and 24 h after seisures). Morphologic changes of the hipp- ocampal neurons were observed by HE staining, while the expressions of NPY in hippocampal tissue were measured by immunohistochemieal techniques. Results Seizure levels and neuronal damage of rats in group D were lower than those in group C. In hippocampal tisse, NPY expression of three subgroups in Group C increased significantly than those in group A ( P 〈 0. 05 ) ; NPY expressions of three subgroups of Group D increased significantly than those in group C ( P 〈 0. 05 ). No statistically different NPY expression was found between group B and group C. Up-regulation of NPY expression was noted from 6 h to 24 h time point after seisures in the same group. Conclu- sion GBE pretreatment has some antiepileptic effects which are concerned with ameriolation of neuronic damage, the mitigation of seizure degree, and the up-regulation of NPY expression.
出处 《安徽医科大学学报》 CAS 北大核心 2013年第8期925-928,共4页 Acta Universitatis Medicinalis Anhui
基金 广西自然科学基金(编号:2011GXNSFA018241) 桂林市科学研究与技术开发计划项目(编号:20110119-1-7)
关键词 银杏叶提取物 红藻氨酸 癫痫 神经肽Y Ginkgo biloba extract kainic acid epilepsy neuropeptide Y
  • 相关文献

参考文献8

  • 1杜向红,赵红卫,李培全.银杏叶提取物对神经系统损伤的保护作用研究进展[J].实用诊断与治疗杂志,2004,18(1):38-40. 被引量:8
  • 2Van ttaay L, Jovanovska V, Morris M J, et al. Focal administra- tion of neuropeptide Y into the S2 somatosensory eortex maximally suppresses absenee seizures in a genetic rat model[ J]. Epilepsia,2012, 53(3) :477 -84.
  • 3陈丽丽,杨晓苏,肖波,李国良,朱丹彤,李静,梁静慧.癫痫大鼠海马中神经肽Y表达的动态观察及其作用探讨[J].中国神经免疫学和神经病学杂志,2004,11(2):76-79. 被引量:4
  • 4Takahashi Y, Tsunashima K, Sadamatsu M, et al. Ahered hipp- ocampal expression of neuropeptide Y, somatostatin, and gluta- mate decarboxylase in lhara's epileptic rats and spontaneously epi- leptic rats[ J]. Neurosci Lett, 2000, 287 (2) : 105 - 8.
  • 5Cardoso A, Freitas-da-Costa P, Carvalho L S, et al. Seizure-in- duced changes in neumpeptide Y-containing cortical neurons: Po- tential role for seizure threshold and epileptogenesis [ J ]. Epilepsy Behav, 2010, 19(4) :559 -67.
  • 6Drexel M, Kirchmair E, Wiesehhaler-Holzl A, et al. Somatostatin and neuropeptide Y neurons undergo different plasticity in parahip- pocampal regions in kainic acid-induced epilepsy [ J ]. J Neuro- pathol Exp Neurol, 2012,71 (4) :312 - 29.
  • 7Fu L Y, van den Pol A N. GABA excitation in mouse hilar neu- ropeptide Y neurons[ J]. J Physiol, 2007, 579 (Pt 2) :445 -64.
  • 8李国强,刘仕勇,周政,陈军花.慢性颞叶癫痫模型鼠海马结构形态学的变化[J].第三军医大学学报,2007,29(2):125-128. 被引量:7

二级参考文献40

  • 1李国强,周政,刘仕勇,杨梅华,安宁,杨辉.侧脑室内注射海仁酸建立慢性大鼠颞叶癫痫模型[J].中国临床康复,2005,9(25):117-119. 被引量:2
  • 2Racine RJ.Modification of seizure activity by electrical stimulation II.Motor seizure[J].Electroencephalogr Clin Neurophysiol,1972,32:781-794.
  • 3McQuiston AR,Petrozzino JJ,Connor JA,et al.Neuropeptide Y1 receptors inhibit N-type calcium currents and reduce transient calcium increases in rat dentate granule cells[J].J Neurosci,1996,16(4):1422-1429.
  • 4Tauck DL,Nadler JV.Evidence of functional mossy fibbed sprouting in hippocampal formation of kainic acid-treated rats[J].J Neurosci,1985,5:1016-1022.
  • 5Marksteiner J,Sperl G,Gruber B,et al.Functional changes in neuropeptide-Y and somatostain-containing neurons by limbic seizures in the rat[J].Neurosci,1992,50(4):831-846.
  • 6Woldbye DP.Antiepileptic effects of NPY on pentylenetetrazole seizures[J].Regul Pept,1998,75-76:279-282.
  • 7Baraban SC,Hollopeter G,Erickson JC.Knock-out reveal a critical antiepileptic role for neuropeptide-Y[J].Neurosci,1997,17:23-28.
  • 8Klapstein GJ,Colmers WF.Neuropeptide-Y suppresses epileptiform activity in rat hippocampus in vitro[J].J Neurophysiol,1997,78:1651-1661.
  • 9Vezzani A,Cirenni G,Rizzi M,et al.Enhanced neuropeptide-Y release in the hippocampus is associated with chronic seizure susceptibility in kainic acid treated rats[J].Brain Res,1994,660(1):138-143.
  • 10Coler WF,Klapstein G,Fournier A,et al.Presynaptic inhibition by neuropeptide-Y in rat hippocampal slice in vitro is mediated by Y2 receptor[J].Br J Pharmacol,1991,102:41-44.

共引文献16

同被引文献58

引证文献3

二级引证文献16

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部