源自合肥地区表观健康鸡的沙门氏菌耐药性与血清型、基因型相关性分析

Analysis of antimicrobial resistance on Salmonella isolated from nonsymptomatic chickens in clinic in Hefei and its serotype and genotype

为了解源自合肥地区表观健康鸡的沙门氏菌耐药性及其与血清型、基因型的相关性,在前期已鉴定血清型和ERIC基因型的基础上,采用K-B纸片法对21株沙门氏菌分离株进行21种抗生素药物敏感试验,利用PCR技术对耐药菌株进行32种耐药基因检测。结果显示,总耐药率为100%(21/21),耐2种以上抗生素的菌株占76.19%(16/21),多重耐药谱为青霉素-氨苄西林-阿莫西林-链霉素-复方新诺明,其中耐受β-内酰胺类抗生素的类别最多,耐药率最高达100%(21/21)。21株沙门氏菌均检出耐药基因,涉及blaPSE、sul1、strA、blaTEM、sul2、tetA、tetB、aph3-Ⅱa和catⅠ9种,其中blaPSE基因的检出率最高,为85.7%(8/21)。可见源自合肥地区表观健康鸡的沙门氏菌对β-内酰胺类抗生素耐药性最强,与其携带blaPSE基因有关;耐药表型测定结果与耐药基因检测结果基本一致;耐药性与具有相同来源的同一血清型、基因型相关。

In order to investigate the relationship between antimicrobial resistance and serotypes,genotypes of Salmonella isolates from apparently healthy chickens in Hefei,the Kirby-Bauer method was applied to determine the sensitivity of 21 strains susceptibility to 21 antibiotics,and PCR technique also was used to detect 32 kinds of drug-resistance genes,based on identified serotypes and ERIC-PCR gene-type.It was found that 100%（21/21）of the Salmonella isolates were resistant to all antibiotics by Drug Susceptibility Test;76.19%（16/21） were resistant to at least two antimicrobials among the 21 isolates,and the dominating multidrug-resistant spectrum was Penicillin G-Ampicillin-Amoxicillin-Streptomycin-Trimethoprim-sulfamethoxazole.It was the most extensive in resistance to β-lactamase antibiotics by 100%（21/21）.Meanwhile,the resistance genes could be detected from all these isolates by PCR,including blaPSE,sul1,strA,blaTEM,sul2,tetA,tetB,aph3-Ⅱa and catⅠ,and blaPSE gene was the most prevalent with the detected rate of 85.7%（8/21）.It is concluded that the stronggest antimicrobial resistance to β-lactamases drugs on Salmonella isolated from apparently healthy chickens in Hefei due to the existence of blaPSE gene;antimicrobial phenotype is also related to the antimicrobial resistance genes;antimicrobial resistance is in relevant with the same sources of the same serotypes and genotypes.