超临界CO_2流体技术萃取当归挥发油的工艺优选

Optimization of Supercritical CO_2 Fluid Extraction Process for Volatile Oil from Angelica sinensis

目的:优选超临界CO2流体萃取当归挥发油的工艺条件。方法:以藁本内酯含量和出油率的综合评分为指标,采用HPLC测定藁本内酯含量,通过L9(34)正交试验考察萃取压力、温度、时间对萃取效果的影响。结果:当归挥发油的最佳萃取工艺为萃取压力30 MPa,萃取温度50℃,萃取时间2 h,CO2流量25 L.h-1,分离釜Ⅰ压力8 MPa,温度50℃,分离釜Ⅱ压力系统尾压,温度35℃。结论:萃取的当归挥发油得率高、质量好,优选的工艺稳定可行。

Objective： To optimize supercritical CO2 fluid extraction process parameters of volatile oil from Angelica sinensis.Method： With composite score of the content of ligustilide and yield of volatile oil as index,the content of ligustilide was determined by HPLC,L9（34） orthogonal test was used to investigate effect of extraction temperature,extraction pressure,extraction time on extraction efficiency.Result： Optimum process parameters were as follows： extraction pressure 30 MPa,extraction temperature 50 ℃,extraction time 2 h,flow rate of CO2 25 L.h-1,pressure of separation kettle I 8 MPa with temperature at 50 ℃,separation pot II pressure of the system tail pressure with temperature at 35 ℃.Conclusion： This optimized process was stable and feasible,which could be adopted to extract volatile oil from A.sinensis with high yield and good quality.