摘要
目的:对土鳖虫多肽进行分离纯化,并对其溶栓活性进行研究。方法:采用仿生酶解工艺制备土鳖虫复合多肽,以溶栓活性为指标,采用Sephadex G-25,SP Sephadex C-25凝胶柱和RP-HPLC C18半制备色谱柱对复合多肽进行分离纯化,并测定目标多肽组分(Peak b)的溶栓活性、肽含量和相对分子质量。结果:目标多肽组分的溶栓活性为814 430 U.mg-1,肽含量为95.7%,相对分子质量分布在3 211～3 547。结论:利用仿生酶解制备的土鳖虫多肽,经分离纯化后,可得到具有较强溶栓活性的多肽组分。
Objective： To purify polypeptide from Eupolyphaga sinensis and investigate its thrombolytic activity.Method： With thrombolytic activity as index,polypeptide was obtained by bionic enzymatic,which was purified by Sephadex G-25,SP Sephadex C-25 and RP-HPLC C18 semi-preparation column.Thrombolytic activity,peptide content and relative molecular weight of target component were determined.Result： Thrombolytic activity of target polypeptide component was 814 430 U.mg-1,peptide content was 95.7% and relative molecular weight was in the range of 3 211-3 547.Conclusion： Polypeptide from E.sinensis could be prepared by bionic enzymatic,and a component of polypeptide with strong thrombolytic activity could be obtained through separation and purification.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第14期53-55,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
山东省自然科学基金项目(ZR2009CL027)
济南市科技局科技明星计划项目(20100118)
山东省医学科学院医学基金项目(201023)
关键词
土鳖虫
多肽
分离纯化
溶栓活性
仿生酶解
Eupolyphaga sinensis
polypeptide
purification
thrombolytic activity
bionic enzymatic
