大孔吸附树脂纯化倒卵叶五加中紫丁香苷的工艺优选

Optimization of Separation and Purification Technology of Syringin in Acanthopanax obouatus with Macroporous Absorption Resin

目的:优选大孔吸附树脂纯化倒卵叶五加中紫丁香苷的工艺条件。方法:采用HPLC测定紫丁香苷含量,通过静态吸附-洗脱试验考察大孔树脂型号,利用单因素试验考察上样量、吸附流速、洗脱机浓度及用量对纯化工艺的影响。结果:选用XDA-1型大孔树脂,其纯化工艺条件为上样量每g树脂吸附3.5 mg紫丁香苷,吸附流速1 BV.h-1,用9 BV 40%乙醇于2BV.h-1流速洗脱,紫丁香苷纯度由5.4%提高到23.1%。结论:XDA-1型大孔树脂可用于纯化倒卵叶五加提取液,紫丁香苷收率和纯度均达满意效果。

Objective： To optimize separation and purification technology of syringin in Acanthopanax obouatus with macroporous absorption resin.Method： With the content of syringin as index,which was determined by HPLC,model of macroporous absorption resin was screened by static adsorption-elution test,purification conditions were investigated by single factor tests.Result： XDA-1 macroporous adsorption resin model was adopted,its purification technology was as following： the amount of sample 3.5 mg syringin with 1 g XDA-1 macroporous absorption resin,adsorption velocity 1 BV.h-1,eluted with 9 BV 40% ethanol at 2 BV.h-1.Purity of syringin was up to 28.14% from 5.47%.Conclusion： XDA-1 macroporous resin could be used to purify extract of A.obouatus,yield and purity of syringin obtained satisfactory results.