猪苓多糖硫酸酯的制备及活性测定

Preparation and Activity Assay of Sulfated Polysaccharides from Polyporus umbellatus

目的:制备猪苓多糖硫酸酯,同时测定其活性。方法:采用醇沉分级、大孔吸附树脂、聚酰胺树脂和脱蛋白法从中药猪苓中提取分离得到猪苓多糖,硫磺酸-吡啶法进行硫酸酯化,经Sephadex G-75、透析得到硫酸化猪苓多糖,并体外观察硫酸酯化多糖对羟基自由基清除作用。结果:猪苓多糖硫酸酯化前后均有清除Fenton反应由Fe2+-H2O2体系产生的羟自由基,硫酸酯化猪苓多糖对羟自由基的清除能力增强,并呈明显量效关系。结论:制备猪苓多糖硫酸酯的方法准确可靠,为猪苓多糖的进一步开发利用提供实验依据。

Objective： To prepare sulfated polysaccharides from Polyporus umbellatus and investigate its anti-oxidation activity.Method： Polysaccharides was extracted and isolated by alcohol precipitation,macroporous resin,polyamide resin,deproteinization from P.umbellatus.Polysaccharides was sulfated by sulfuric acidpyridine method,sulfated polysaccharides was depurated by Sephadex G-75 and dialysis,and in vitro scavenging effect of sulfated polysaccharides for hydroxyl free radical was observed.Result： Before and after sulfated,polysaccharides from P.umbellatus showed good antioxidation in scavenging hydroxyl free radical from Fe2 ＋-H2O2 system,scavenging capacity of sulfated polysaccharides for hydroxyl free radical enhanced,and showed a clear dose-effect relationship.Conclusion： This method of preparing sulfated polysaccharides was accurate and reliable,it could provide references for further development and utilization of polysaccharides from P.umbellatus.