半边风苯丙素类成分及抑制中性粒细胞呼吸爆发活性研究

Isolation and Anti-PMN Respiratory Burst Activity of Phenylpropanoids Compounds from Dendropanax chevalieri

目的:继续对半边风的化学成分进行研究,并对包括前期研究的9个苯丙素类化合物进行生物活性评价,为半边风在畲族医药中的临床应用提供初步的药理学依据。方法:利用各种柱色谱对半边风根95%乙醇提取物正丁醇部位的化学成分进行分离纯化,根据化合物的理化性质及光谱数据鉴定其结构。采用化学发光法评价9个苯丙素类成分对大鼠中性粒细胞(PMN)呼吸爆发的抑制活性。结果:分离了4个苯丙素类化合物,分别鉴定为芥子醛葡萄糖苷(1)、丁香苷(2)、松柏醛葡萄糖苷(3)、淫羊藿苷E5(4)。在药理活性研究中,有6个苯丙素化合物对大鼠PMN呼吸爆发表现出不同的活性,其活性大小依次为东莨菪素>右旋松脂素>右旋丁香树脂酚>松柏醛>β-hydroxypropiovanillon>维生素C(VC)>淫羊藿苷E5,而芥子醛葡萄糖苷、丁香苷和松柏醛葡萄糖苷则对PMN呼吸爆发没有抑制作用。结论:化合物3为首次从该属植物中分离得到。半边风中的苯丙素类化学成分具较强的抗PMN呼吸爆发作用,酚羟基可能是苯丙素类化合物抑制大鼠PMN呼吸爆发的重要基团。

Objective： To study the phenylpropanoids chemical constituents from the root of Dendropanax chevalieri,and evaluate the biological activity of the nine phenylpropanoids including the five phenylpropanoids isolated previously,and to provide preliminary pharmacological basis for the clinical application of the root of D.chevalieri in medicine of She nationality.Method： The constituents were isolated from n-butanol extract of D.chevalieri 95% ethanol extract by all kinds of column chromatography.Their structures were elucidated by analysis of physical chemical properties and spectral data.By using phorbol myristate acetate（PMA） as the stimulant,luminol-dependent chemiluminescence was used to compare the effects of the nine phenylpropanoids compounds on the respiratory burst of rat neutrophils.Result： Four phenylpropanoid compounds,sinapaldehye glucoside（1）,syrigin（2）,coniferaldehyde 4-O-β-D-glucopyranoside（3） and icariside E5（4）,were isolated and identified from n-butanol extract.As results of pharmacological studies,six phenylpropanoid compounds revealed different inhibitory activity on the respiratory burst of rat neutrophils.Their activity were ranked in the decreasing order of scopoletin （＋）-pinoresinol （＋）-syringaresinol coniferaldehyde β-hydroxypropiovanillon VC icariside E5,while sinapaldehye glucoside,syrigin and coniferaldehyde 4-O-β-D-glucopyranoside had no inhibitory activity.Conclusion： Compound 3 is isolated from this genus for the first time.Phenylpropanoid compounds isolated from the root of D.chevalieri has a strong anti-PMN respiratory burst activity and hydroxyl group might be a pharmacophore.This is the fist report of the pharmacological evidence for the clinical application of the root of D.chevalieri in medicine of She nationality.