HPLC测定藏药樱草杜鹃中金丝桃苷、槲皮苷和槲皮素的含量

Determination of Hyperosid,Quercetrin and Quercetin in Rhododendron Primulaeflorum by HPLC

目的:建立藏药樱草杜鹃中金丝桃苷、槲皮苷和槲皮素含量的HPLC测定方法,为药材质量控制与评价提供参考。方法:采用HPLC,Welch Ultimate XB-C18色谱柱(4.6 mm×250 mm,5μm),柱温30℃,流速1.0 mL.min-1,进样量10μL,检测波长350 nm,流动相A乙腈-甲醇(5∶1)-B 0.1%甲酸,二元梯度洗脱。结果:3种成分在70 min内达到良好分离,金丝桃苷、槲皮苷和槲皮素线性范围分别为4.41～88.20 mg.L-1(r=0.999 6),2.94～58.80 mg.L-1(r=0.9996),1.485～29.70 mg.L-1(r=0.999 6);加样回收率分别为103.37%(RSD 1.67%),98.07%(RSD 1.41%),100.19%(RSD 1.20%)。结论:方法操作简单、结果准确、重复性较好,可作为藏药樱草杜鹃质量控制的有效方法之一。

Objective： To establish an HPLC method for determining the content of hyperoside,quercitrin and quercetin in Rhododendron Primulaeflorum,and to lay the foundation for the quality control and / or quality evalution of R.primulaeflorum.Method： A high-performance liquid chromatography equipped a Welch Ultimate XB-C18（4.6 mm ×250 mm,5 μm） column with UV detection was used.The mobile phase consisted of acetonitrile and methanol（5∶ 1）（A） and 0.1% formic acid in water（B） with gradient elution.The column temperature was kept at 30 ℃ and the flow rate was 1.0 mL.min-1.The detection wavelength was set at 350 nm.Result： The good separation of three compounds was achieved within 70 min.The linear range of hyperoside,quercitrin and quercetin were 4.41-88.20 mg.L-1（r =0.999 6）,2.94-58.80 mg.L-1（r =0.999 6）,1.48529.70 mg.L-1（r = 0.999 6） respectively.The average recovery were 103.37%（RSD 1.67%）,98.07%（RSD 1.4%）,100.19%（RSD 1.20%） respectively.Conclusion： This method is simple,accurate with good reproducibility and stability.It can be used as effective methods for quality control of R.primulaeflorum.