HPLC测定莲栀清火胶囊中栀子苷、穿心莲内酯和脱水穿心莲内酯的含量

HPLC Determination of Geniposide,Andrographolide and Dehydroandrographolide in Lianzhi Qinghuo Capsules

目的:建立高效液相色谱法同时测定莲栀清火胶囊中栀子苷、穿心莲内酯及脱水穿心莲内酯含量的方法。方法:ZORBAX SB-C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-水梯度洗脱,检测波长254 nm,流速1.0 mL.min-1。结果:栀子苷在0.075 8～2.274 0μg(r=1)线性关系良好,平均回收率99.73%,RSD 1.4%;穿心莲内酯在0.046 1～1.383 0μg(r=1)线性关系良好,平均回收率100.51%,RSD 1.2%;脱水穿心莲内酯在0.040 9～1.227 0μg(r=1)线性关系良好,平均回收率为101.19%,RSD 1.2%。结论:方法简便、快速、重复性好,可用于莲栀清火胶囊的质量控制。

Objective： To establish an HPLC method for the simultaneous determination of geniposide,andrographolide and dehydroandrographolide in Lianzhi Qinghuo capsules.Method： Geniposide,andrographolide and dehydroandrographolide were separated on ZORBAX SB-C18（4.6 mm ×250 mm,5 μm） column and detected at 254 nm.The mobile phase was methanol-water with gradient elution.The flow rate was 1.0 mL.min-1.Result： Geniposide,andrographolide and dehydroandrographolide were linear within the range of 0.075 8-2.274 0 μg（r = 1）,0.046 1-1.383 0 μg（r = 1） and 0.040 9-1.227 0 μg（r = 1） respectively.The average recovery was 99.73%（RSD 1.4%）,100.51%（RSD 1.2%） and 101.19%（RSD 1.2%）.Conclusion： The method is simple,reproducible,and it can be used for the quality control of Lianzhi Qinghuo capsules.