二苯乙烯苷在小鼠行为模型上的抗抑郁作用

Study on Anti-depressant Effects of 2,3,5,4'-tetrahydroxy-stilbene-2-O-β-D-glucoside

目的:探讨2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷(THSG)的抗抑郁作用及其机制。方法:雄性昆明种小鼠随机分为空白对照组,盐酸氟西汀组(5 mg.kg-1)和THSG组(200,100,50 mg.kg-1),连续ig给药7 d后,进行小鼠悬尾试验(TST)和小鼠强迫性游泳试验(FST);除空白对照组外,各组连续ig给药7 d后,分别ip利血平(1 mg.kg-1),观察THSG对利血平所致小鼠眼睑下垂和体温下降的拮抗作用。结果:在小鼠悬尾试验和小鼠强迫性游泳试验中,THSG高、中剂量(200,100 mg.kg-1)均能明显缩短小鼠在悬尾实验和强迫游泳实验中的不动时间(P<0.01)。在利血平诱导小鼠抑郁试验中,THSG高、中剂量(200,100 mg.kg-1)均能显著对抗利血平诱导的小鼠体温下降和眼睑下垂(P<0.01,P<0.05)。结论:THSG具有明显的抗抑郁作用,其作用机制可能与单胺递质有关。

Objective： To investigate the antidepressant effect and the possible mechanism of 2,3,5,4＇-tetrahydroxy-stilbene-2-O-β-D-glucoside（THSG） in animal model.Method： Mail KM mice were randomly divided into 5 groups： control group（normal saline）,fluoxetine group（5 mg.kg-1）,THSG groups（200,100,50 mg.kg-1）.After oral administration for 7 days,the tail-suspending test（TST）,the forced swimming test（FST） and reserpine-antagonism test were carried out to evaluate the antidepressant effects of THSG.In particular,reserpine（1 mg.kg-1） was given by intrapertioneal injection before the reserpine-antagonism test.Result： Compared with the control group,the immobility time of TST and FST was shorted in THSG high,and middle,dose group（P 0.01）.Compared with the model group,THSG high,middle,dose group could significantly reverse the ptosis,and decrease body temperature induced by reserpine in mice（P 0.01,P 0.05）.Conclusion： THSG has significant antidepressant effect,and its mechanism may be related to monoamine neurotransmitters.