罗汉果黄酮对运动大鼠心肌能量代谢酶及PPARα mRNA表达的影响

Effects of Momordica Grosvenori Flavones on Myocardial Energy Metabolism Enzymes and Expression of PPARα mRNA in Exercise Rats

目的:研究运动前补充罗汉果黄酮(MGF)对力竭运动大鼠心肌能量代谢酶和过氧化物酶体增殖剂活化受体α(PPARα)mRNA表达的影响,以初步探讨其作用机制。方法:SD雄性大鼠50只,随机分为5组,每组10只,分别为安静对照组(NC),运动对照组(ME),运动+ig低剂量罗汉果黄酮组(MGFL),运动+ig中剂量罗汉果黄酮组(MGFM),运动+ig高剂量罗汉果黄酮组(MGFH)。每天在运动前30 min ig 1次,连续ig 6周,6 d/周,低、中、高3组的ig剂量分别为100,200,400mg.kg-1,对照组ig等量生理盐水。力竭训练结束后测定大鼠心肌组织肌酸激酶(CK)、乳酸脱氢酶(LDH)和琥珀酸脱氢酶(SDH)活性,逆转录聚合酶链式反应(RT-PCR)测定心肌组织PPARαmRNA表达。结果:力竭游泳运动引起大鼠心肌CK活性下降,运动对照组显著低于安静对照组(P<0.01),MGF低剂量组低于安静对照组(P<0.05),但MGF各组均显著高于运动对照组(P<0.01)。LDH活性在力竭游泳运动组显著低于安静对照组(P<0.01),在MGF各组均高于运动对照组(P<0.05或P<0.01)。SDH活性在力竭游泳运动组显著低于安静对照组(P<0.01),MGF低剂量组低于安静对照组(P<0.05),MGF中、高剂量组高于运动对照组(P<0.05)。运动对照组PPARαmRNA表达水平显著低于安静对照组(P<0.01),MGFL组PPARαmRNA表达水平高于运动对照组(P<0.05),在MGF中、高剂量组PPARαmRNA表达水平均显著高于运动对照组(P<0.01)。结论:补充罗汉果黄酮能够提高能量代谢酶CK,LDH,SDH的活性和上调PPARαmRNA表达水平,对改善机体能量代谢和保护心肌组织的过度损伤具有良好的作用。

Objective： To study the effects and mechanism of momordica grosvenori flavones（MGF） on myocardial energy metabolism and expression of peroxisome proliferators-activated receptor α（PPARα） mRNA in exercise rats.Method： Fifty healthy male Sprague-Dawley rats were randomly divided into five groups（n = 10） ： quiet control group（NC）,exercise control group（ME）,exercise ＋ ig low dose flavones group（MGFL）,ig moderate dose flavones group（MGFM） and intragastric gavage（ig） high dose flavones group（MGFH）.All rats in administrated groups were intragastric gavaged in profession once time at half an hour before exercise.The administration was continued for 6 d / week in total 6 weeks at the dose of 100,200,400 mg.kg-1 in low,medium and high dose group respectively,while the rats in unadministrated groups were treated by physiological saline solution according to the same dose.The rats were killed immediately by decapitation at the end of training.The activities of creatine kinase（CK）,lactate dehydrogenase（LDH） and succinate dehydrogenase（SDH） in myocardial tissue were measured according the corresponding kit method respectively.The expression of PPARαmRNA in myocardial tissue was determined reverse transcription polymerase chain reaction（RT-PCR）.Result： The activity of myocardial CK was decreased in rat by exhaustive swimming exercise.It was significantly lower in exercise control group than that in quiet control group（P 0.01）.There was significant difference in low dose MGF group compared with quiet control group（P 0.05）.The activity of myocardial CK was obviously higher in MGF groups than that in exercise control group（P 0.01）.The activity of LDH was significantly lower in exhaustive swimming groups than that in quiet control group（P 0.01）.It was higher in each MGF group than that in exercise control group（P 0.05 or P 0.01）.The activity of SDH was obviously lower in exhaustive swimming groups than that in quiet control group（P 0.01）.It was lower in low dose MGF group than that in quiet control group（P 0.05）,while it was higher in moderate,and high dose MGF groups than that in exercise control group（P 0.05）.The expression of PPARα mRNA was obviously lower in exercise control group than that in quiet control group（P 0.01）.It was higher in MGFL group than that in exercise control group（P 0.05）.The expression of PPARα mRNA in MGFM and MGFH groups were significantly higher than that in exercise control group（P 0.01）.Conclusion： Supplement of momordica grosvenori flavones could improve the activities of energy metabolic enzymes CK,LDH and SDH,and upregulate the expression of PPARα mRNA.This has a good role in improving the energy metabolism and protecting the myocardial tissue from overuse exercise injury.