去甲乌药碱与6-姜酚对心肌细胞的正性肌力作用

Positive Inotropic Effect of Higenamine and 6-gingerol on Cardiac Myocytes

目的:研究去甲乌药碱(higenamine)与6-姜酚(6-gingerol)单体及其配伍在正常心肌细胞的正性肌力作用。方法:急性分离成年SD大鼠左心室肌细胞,分别使用不同单体和单体间不同浓度的相互配伍作用于心肌细胞,使用Ion Optix单细胞收缩与离子测量系统检测细胞的最大收缩舒张速率(±dp/dt)、收缩幅度(ph/bl)、50%收缩舒张时间常数(T50S,T50D)的变化。结果:0.03～1μmol.L-1去甲乌药碱能够浓度依赖性增强心肌收缩力和最大收缩舒张速率,起效浓度为0.1μmol.L-1,1μmol.L-1能到最大效应,其使收缩幅度增加为234%,最大收缩速率增加为219%,最大舒张速率增加为272%,其对50%收缩舒张常数无影响,EC50约为0.205μmol.L-1,>1μmol.L-1即产生心肌毒性作用。3～30μmol.L-16-姜酚对心肌细胞收缩功能无显著影响,大于30μmol.L-1使心肌细胞产生不节律收缩。相互配伍不能增强去甲乌药碱的强心作用。结论:去甲乌药碱具有强心作用,与6-姜酚配伍不能显著增强去甲乌药碱的强心作用。

Objective： To study the positive inotropic effect of higenamineand 6-gingerol and their compatibility on normal cardiac myocytes.Method： The left ventricular cardiac myocytes were isolated from normal rat by traditional Langendroff method.The cardiac myocytes contraction were detected by using different monomers and their compatibility（Ion Optix cell dimensioning systems）.The detections included the maximum velocity of systolic and diastolic（± dp / dt）,contraction amplitude（ph / bl） and the time course constant of 50% systolic and diastolic（T50S,T50D）.Result： 0.03-1 μmol.L-1 higenamine enhances ± dp / dt,ph / bl with a dosedependent manner,but not influenced the T50S and T50D.The minimum effective concentration was 0.1 μmol.L-1,EC50 was approximately 0.205 μmol.L-1,and 1 μmol.L-1 could get the maximum efficacy.The maximum efficacy of ph / bl,＋ dp / dt,-dp / dt was up to 234%,219% and 272%.It＇s could induce the arrhythmia contraction when the dose was higher than 1 μmol.L-1.3-30 μmol.L-1 6-gingerol had not any effects of contractility on cardiac myocytes.However,the higher concentrations than 30 μmol.L-1 also induced the arrhythmia contraction.This research also investigated the 3,10 μmol.L-16-gingerol compatibility with 0.1 μmol.L-1 and 0.3 μmol.L-1 higenamine.Mutual compatibility could not enhance the positive inotropic effect of higenamine.Conclusion： Higenamine plays the positive inotropic effect,and 6-gingerol has nether any effects on monomer,nor compatibility with higenamine.