参附注射液减轻血栓闭塞性脉管炎模型大鼠损伤的作用研究

Mechanism of Shenfu Injection Alleviating Lesions in Experimental Buerger's Disease Model Rats

目的:观察参附注射液(Shenfu injection,SF)对血栓闭塞性脉管炎(thromboangitis obliterans,TAO)模型大鼠的防治作用及其机制。方法:健康成年雄性SD大鼠,随机分为5组,即假手术组、TAO模型组、SF低、中、高剂量(2.5,5,10 mg.kg-1)组。采用月桂酸股动脉注射制作大鼠TAO模型。经尾静脉给予生理盐水或低、中和高剂量SF,连续15 d。观察大鼠的体征变化、病理切片血栓分级、测定血小板数量、血浆血栓素B2(thromboxane B2,TXB2)、6-酮基-前列腺素F1α(6-keto-prostaglandin F1α,6-K-PGF1α)含量等指标。结果:SF明显抑制血管内血栓形成;SF中,高剂量组血小板计数较模型组显著减少(分别为P<0.05和P<0.01)。另外,SF中、高剂量组血浆TXB2也明显减少(P<0.01)而6-K-PGF1α显著增加(P<0.01)。结论:SF可能通过其加强对血小板聚集的抑制作用,提高血管的抗血栓功能,减少TAO模型大鼠血栓形成,以及改善其病变体征。

Objective： To explore the mechanism and the therapeutic effects of Shenfu injection（SF） on the rat models with buerger＇s disease or thromboangiitis obliterans（TAO）.Method： Adult male Sprague Dawley rats were randomly divided into sham operated group,TAO model group,SF low dose（2.5 mg.kg-1）,medium dose（5 mg.kg-1） and high dose（10 mg.kg-1） groups（n =8）.Rats were intravenously administered SF 2.5,5,10 mg.kg-1 or saline once per day for 15 days.TAO model was prepared by injecting sodium laurate into the femoral artery of rats.Then we examined the alterations of pathological signs,pathologic grading of thrombus,blood platelet count,the contents of thromboxane B2（TXB2） and 6-keto-prostaglandin F1α（6-K-PGF1α） in plasma following SF or saline treatment.Result： More physiological signs of lesions and higher grades of pathological thrombosis,the increase in the TXB2 content,blood platelet count and the decrease of 6-K-PGF1α content in TAO model group were observed in our experiments;Compared with TAO model group,SF treatment significantly improved the physiological signs of lesions induced by sodium laurate injection and reduced the numbers of thrombus formation and the TXB2 content,as well as blood platelet count but increased the 6-K-PGF1α content.Conclusion： These results first suggest that SF can cause a significant therapeutic effect on experimental TAO model rats by its inhibiting platelet aggregation and enhancing anti-thrombus function of vessel endothelia.