上中下通用痛风汤治疗高尿酸血症的实验研究

Experimental Study on Upper Middle Lower Generic Gout Decoction in Hyperuricemia

目的:探讨上中下通用痛风汤治疗高尿酸血症的作用。方法:采用腺嘌呤和乙胺丁醇联用,ig,连续21 d,建立高尿酸血症大鼠模型,上中下通用痛风汤高、中、低剂量组分别给予上中下通用痛风汤5,10,20 g.kg-1,ig,连续给药21 d后检测血清尿酸(UA)、黄嘌呤氧化酶(XOD)、腺苷脱氨酶(ADA)、尿素氮(BUN)、肌酐(Cre)水平。结果:与模型组大鼠血清UA(310.12±22.23)μmol.L-1比较,上中下通用痛风汤高、中、低剂量组,分别为(188.99±20.82),(202.56±19.77),(226.53±29.83)μmol.L-1水平显著降低(P<0.05),且其降低UA效果高于别嘌呤醇组(P<0.05);上中下通用痛风汤中剂量组XOD含量为(40.15±0.74)U.L-1,明显低于模型组(49.19±1.61)U.L-1(P<0.05);上中下通用痛风汤高、中剂量组ADA含量为(24.02±0.32),(28.61±1.78)U.L-1,明显低于模型组(34.36±3.73)U.L-1(P<0.05);上中下通用痛风汤高、中、低剂量组血清BUN及Cre水平,显著低于模型组(P<0.05)。结论:上中下通用痛风汤具有显著的抗高尿酸血症的作用,可显著降低血尿酸水平和降低尿酸生成,并对肾脏亦有保护作用。

Objective： To explore the effect of Upper middle lower generic gout decoction in the treatment of hyperuricemia.Method： Hyperuricemia rat model was established by administration of adenine and ethambutol,ig,for 21 days.High,middle,low dose groups were given upper middle lower generic gout decoction 5,10,20 g.kg-1continuously for 21 days,ig respectively.The concentration of uric acid（UA）,xanthine oxidase（XOD）,adenosine deaminase（ADA）,blood urea nitrogen（BUN） and creatinine（Cre） were detected in serum.Result： The content of UA of high,middle,low dose groups were（188.99 ± 20.82）,（202.56 ± 19.77）,（226.53 ± 29.83） μmol.L-1respectively,which was significantly lower than those of the model group（310.12 ±22.23） μ mol.L-1,（P 0.05）,also distinctly lower than that of allopurinol group（P 0.05）.The XOD content of middle group was（40.15 ± 0.74） U.L-1,which was significantly lower than that in the model group（49.19 ± 1.61） U.L-1,（P 0.05）.ADA content of high and middle dose groups（24.02 ± 0.32）,（28.61 ±1.78） U.L-1,respectively were obviously lower than those in the model group（34.36 ±3.73） U.L-1,（P 0.05）.Both BUN and Cre content of all the three dosage groups were significantly lower than those in the model group（P 0.05）.Conclusion： Upper middle lower generic gout decoction show better anti-hyperuricemia effect,and also significantly reduce the BUN level and downregulate the production of UA,meanwhile,it exerts protective effects on the kidney.